Atmospheric pressure radio frequency (rf) glow discharge characteristics were studied aiming for the plasma treatment of living tissues such as pork and human skin. Electrical and optical measurements of the plasma gave reasonable values at low current of 4 mA and gas temperature of 60 degrees C at 100 W. The colorimetric measurement of the treated pork sample demonstrated negligible thermal effect from the plasma. In addition, the sterilization efficiency of Escherichia coli inoculated on pork surface was comparable with that of a conventional UV sterilizer. The experimental results promise that the atmospheric pressure rf plasma can be safely applied to human skin treatment without electrical and thermal damage. (C) 2009 Elsevier B.V. All rights reserved.