Au/TiO(2)/Ti electrodes have been prepared by galvanic deposition of gold particles on TiO(2) nanotube substrates. Titanium oxide nanotubes are fabricated by anodizing titanium foil in a Dimethyl Sulfoxide electrolyte containing fluoride. The scanning electron microscopy results indicated that gold particles are homogeneously deposited on the surface of TiO(2) nanotubes. The TiO(2) layers consist of individual tubes of about 40-80 nm diameters. The electro-catalytic behavior of Au/TiO(2)/Ti and flat gold electrodes for the ascorbic acid electro-oxidation was studied by cyclic voltammetry. The results showed that the flat gold electrode is not suitable for the oxidation of ascorbic acid. However, the Au/TiO(2)/Ti electrodes are shown to possess catalytic activity toward the oxidation reaction. Catalytic oxidation peak current showed a linear dependence on the ascorbic acid concentration and a linear calibration curve is obtained in the concentration range of 1-5 mM of ascorbic acid. Also, determination of ascorbic acid in real samples was evaluated. The obtained results were found to be satisfactory. Finally the effects of interference on the detection of ascorbic acid were investigated. (C) 2011 Elsevier B.V. All rights reserved.