Effect of Demineralized Bone Particles (DBP) on Cell Growth and ECM Secretion in PLGA/DBP Hybrid Scaffold for Cartilage Tissue Engineering

Hyoung Eun Kim; Ha Neul Kim; Hyun Yu; Jeong Eun Song; Seoun Yeoung Jeoung; Youngun Kim; Dongwon Lee; Gilson Khang

Macromolecular Research, Vol.20, No.10, 1044-1053, October, 2012

DOI10.1007/s13233-012-0148-5 Export Citation

Effect of Demineralized Bone Particles (DBP) on Cell Growth and ECM Secretion in PLGA/DBP Hybrid Scaffold for Cartilage Tissue Engineering

Hyoung Eun Kim, Ha Neul Kim, Hyun Yu, Jeong Eun Song , Seoun Yeoung Jeoung, Youngun Kim, Dongwon Lee^†, and Gilson Khang^†

Department of BIN Fusion Tech, Polymer Fusion Resarch Center and Dept of Polymer·Nano Sci Tech, Chonbuk National University, Jeonbuk, 561-756, Korea

E-mail:,

In this study, we investigated the effect of deminerized bone particles (DBP) on the regeneration of chondrocyte in poly(lactic-co-glycolic acid) (PLGA)/DBP hybrid scaffolds. PLGA/DBP hybrid scaffolds were designed by varying the DBP content with 0, 10, 20, 40, and 80 w/t% and manufactured by a solvent casting/saltleaching method. We evaluated the potential of PLGA/DBP hybrid scaffolds for cartilage regeneration. Chondrocytes of New Zealand white rabbits were seeded on PLGA/DBP hybrid scaffolds. MTT assay, glycosaminoglycan (GAG) assay, Hematoxylin-Eosin (H&E) staining, Safrainin-O staining, and reverse transcription polymerase chain reaction (RT-PCR) were performed to confirm the effect of the DBP on cell proliferation and extracellular matrix (ECM) production. As the DBP content of these scaffolds became higher, the rate of cell growth and the amount of GAG increased. The PLGA/DBP hybrid scaffolds with DBP content showed better cell proliferation, stable in vitro tissue morphology, superior cartilaginous tissue formation, and sGAG production. In conclusion, PLGA/DBP hybrid scaffolds promote the potential cell delivery vehicle and act as the structural basis for cartilage tissue engineering.

Keywords:chondrocyte;poly(lactic-co-glycolic acid) (PLGA);demineralized bone particle (DBP).

[References]

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Madihally SV, Matthew HWT, Biomaterials., 20, 1133 (1990)
Khang G, Lee SJ, Kim MS, Lee HB, in Encyclopedia of Medical Devices and Instrumentation, 2nd ed., Wiley Press, New York, 366 (2008)
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Yoshioka T, Kawazoe N, Tateishi T, Chen G, Biomaterials., 29, 3438 (2008)
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Khang G, Lee SJ, Han CW, Rhee JM, Lee HB, Adv. Exp. Med. Biol., 657, 235 (2003)
Liao CJ, Chen CF, Chen JH, Chiang SF, Lin YJ, Chang KY, J. Biomed. Mat. Res., 59, 676 (2002)
Munirah S, Kim SH, Idrus RBH, Khang G, J. Orthop. Surg. Res., 3, 17 (2008)
Lee JH, Park SJ, Chun HJ, Kim CH, Int. J. Tissue Reg., 1, 1 (2010)
Sung HJ, Meredith C, Johnson C, Galis ZS, Biomaterials., 25, 5735 (2004)
Lu H, Hoshiba T, Kawazoe N, Chen G, Biomaterials., 32, 2489 (2011)
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Mizuno S, Lycette C, Quinto C, Glowacki J, Mater.Res. Soc. Symp. Proc., 252, 133 (1992)
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Yoon SJ, Kim SH, Ha HJ, Ko YK, So JW, Kim MS, Yang YI, Khang G, Rhee JM, Lee HB, Tissue Eng. Part A., 14, 539 (2008)
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Urist MR, Science., 150, 893 (1965)
Xia W, Liu W, Cui L, Liu Y, Zhong W, Liu D, Wu J, Chua K, Cao Y, J. Bio. Mater. Res. Appl., 71B, 373 (2004)
Gutierrez MC, Garcia-Carvajal ZY, Jobbagy M, Rubio T, Yuste L, Rojo F, Ferrer ML, del Monte F, Adv. Funct. Mater., 17(17), 3505 (2007)
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Rossella D, Claudia C, Ida G, Tiziana M, Bice C, Polym. Degrad. Stab., 95, 694 (2010)
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Yen HJ, Tseng CS, Hsu SH, Tsai CL, Biomed. Microdevices., 11, 615 (2009)
Baek CH, Lee JC, Jung YG, Ko YJ, Yoon JJ, Park TG, The Laryngoscope., 112, 1050 (2002)
Shuanhu Z, Karen EY, Karim E, Julie G, Cell Tissue Banking., 6, 33 (2005)
Glowacki J, Yates K, Little G, Mizuno S, Mater. Sci. Eng., C6, 199 (1998)

[Referenced By]

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[2] Klaus EK, Clinical Biochemistry., 25, 155 (1994)

[3] Madihally SV, Matthew HWT, Biomaterials., 20, 1133 (1990)

[4] Khang G, Lee SJ, Kim MS, Lee HB, in Encyclopedia of Medical Devices and Instrumentation, 2nd ed., Wiley Press, New York, 366 (2008)

[5] Weiner AA, Int. J. Pharm., 360, 107 (2008)

[6] Murphy WL, Peters MC, Kohn DH, Mooney DJ, Biomaterials., 21, 2251 (2000)

[7] Hu SG, Jou CH, Yang MC, Biomaterials., 24, 2685 (2003)

[8] Munirah S, Kim SH, Ruszymah BHI, Khang G, Eur. Cell. Mater, 15, 41 (2008)

[9] Oh SH, Kang SG, Lee JH, J. Mater. Sci. Mater Med., 17, 131 (2006)

[10] Yoshioka T, Kawazoe N, Tateishi T, Chen G, Biomaterials., 29, 3438 (2008)

[11] Shum AWT, Li J, Mak AFT, Polym. Degrad. Stab., 87, 487 (2005)

[12] Khang G, Lee SJ, Han CW, Rhee JM, Lee HB, Adv. Exp. Med. Biol., 657, 235 (2003)

[13] Liao CJ, Chen CF, Chen JH, Chiang SF, Lin YJ, Chang KY, J. Biomed. Mat. Res., 59, 676 (2002)

[14] Munirah S, Kim SH, Idrus RBH, Khang G, J. Orthop. Surg. Res., 3, 17 (2008)

[15] Lee JH, Park SJ, Chun HJ, Kim CH, Int. J. Tissue Reg., 1, 1 (2010)

[16] Sung HJ, Meredith C, Johnson C, Galis ZS, Biomaterials., 25, 5735 (2004)

[17] Lu H, Hoshiba T, Kawazoe N, Chen G, Biomaterials., 32, 2489 (2011)

[18] Mogami H, Urist MR, J. Cell. Biol., 62, 510 (1974)

[19] Mizuno S, Lycette C, Quinto C, Glowacki J, Mater.Res. Soc. Symp. Proc., 252, 133 (1992)

[20] Nehrer S, Breinan HA, Ramappa A, Spector M, Biomaterials., 18, 769 (1997)

[21] Mizuno S, Glowacki J, Biomaterials., 17, 1819 (1996)

[22] Vilalta M, Jorgensen C, Degano IR, Chernajovsky Y, Gould D, Noel D, Andrades JA, Becerra J, Rubio N, Blanco J, Biomaterials., 30, 4986 (2009)

[23] Munirah S, Yoon SJ, Ko YK, Ha HJ, Kim SH, So JW, Idrus RBH, Khang G, J. Biomater. Sci., Polym. Edn., 19, 1219 (2008)

[24] Ko YK, Kim SH, Jeong JS, Ha HJ, Yoon SJ, Rhee JM, Kim MS, Lee HB, Khang G, Polym.(Korea), 31(1), 14 (2007)

[25] Song Y, Yoo H, Eum S, Kim OY, Yoo SC, Kim HE, Lee D, Khang G, Polym.(Korea), 35(3), 189 (2011)

[26] Lee SJ, Lee I, Lee YM, Lee HB, Khang G, J. Biomater. Sci., Polym. Ed., 15, 1003 (2004)

[27] Jang JW, Han CW, Kim MS, Cho SH, Lee HB, Khang G, Polym. Korea., 28., 382 (2004)

[28] Yoon SJ, Kim SH, Ha HJ, Ko YK, So JW, Kim MS, Yang YI, Khang G, Rhee JM, Lee HB, Tissue Eng. Part A., 14, 539 (2008)

[29] Lee SK, Hong HK, Kim SJ, Kim YK, Song YS, Ha Y, Lee D, Khang G, Polym.(Korea), 34(5), 398 (2010)

[30] Urist MR, Science., 150, 893 (1965)

[31] Xia W, Liu W, Cui L, Liu Y, Zhong W, Liu D, Wu J, Chua K, Cao Y, J. Bio. Mater. Res. Appl., 71B, 373 (2004)

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[35] Linbo W, Jiandong D, Biomaterials., 25, 5821 (2004)

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[37] Yang F, Cui W, Xiong Z, Liu L, Bei J, Wang S, Polym. Degrad. Stab., 91, 3065 (2006)

[38] Gopferich A, Biomaterials., 17, 102 (1996)

[39] Yen HJ, Tseng CS, Hsu SH, Tsai CL, Biomed. Microdevices., 11, 615 (2009)

[40] Baek CH, Lee JC, Jung YG, Ko YJ, Yoon JJ, Park TG, The Laryngoscope., 112, 1050 (2002)

[41] Shuanhu Z, Karen EY, Karim E, Julie G, Cell Tissue Banking., 6, 33 (2005)

[42] Glowacki J, Yates K, Little G, Mizuno S, Mater. Sci. Eng., C6, 199 (1998)