A simple way to form an interpenetrating hydrogel (IPH) by combining a layer-by-layer polyelectrolyte membrane with agarose is reported. The formed IPH membrane is more robust and easily manipulated compared to a polyelectrolyte membrane with the same number of layers. The IPH has good diffusion characteristics and retains the advantageous surface charge from the polyectrolyte composition that facilitates the adsorption of a stable lipid bilayer. The stable adsorption of a lipid bilayer on the IPH creates a biomimetic membrane system that is optimized for utilization in a diffusion chamber.