The adsorption of glucose oxidase (GOD) on graphene oxide (GO) nanoparticles without using any cross-linking reagents and/or additional surface modification was studied. Results of Fourier-transform infrared and ultraviolet-visible absorption spectroscopy confirmed that GOD was successfully immobilized on GO surface. The obtained immobilized GOD showed a wide range of pH stability and improved thermal and storage stability. In addition, GO exhibited good biocompatibility, which has potential advantages for biomedical and clinical diagnosis applications.