The influence of salts and organic nitrogen sources on the growth and acid production of Anaerobiosprillum succiniciproducens was studied. In shake flask experiments, maximum specific growth rate and acid production increased by reducing the salt concentration. Additionally, growth and acid production were affected by the type of organic nitrogen source and by the pH value. Through the optimization, optical density could be increased from 1.9?+/-?0.1 to 3.0?+/-?0.1 and succinic acid concentration from 4.0?+/-?0.2 to 6.5?+/-?0.1?g?L1. In fed-batch experiments with the optimized medium, the final succinic acid concentration was 25.6?% higher compared to the reference. Also, the succinic acid/acetic acid ratio was affected by the optimization. The experimental results indicated that A. succiniciproducens consumed only organic nitrogen sources because growth and productivity were not influenced by the addition of ammonium sulfate.