Chemistry Letters, Vol.41, No.7, 689-690, 2012
One-pot Chemoenzymatic Route to Chitoheptaose via Specific Transglycosylation of Chitopentaose-Oxazoline on Chitinase-template
One-pot regio- and stereospecific synthesis of chitoheptaose ((GlcNAc)(7)) has been achieved by using chitopentaose ((GlcNAc)(5)) and chitobiose ((GlcNAc)(2)) as starting materials. The key intermediate, 1,2-oxazoline derivative of (GlcNAc)(5), could be directly prepared in water and successfully transglycosylated to (GlcNAc)(2) catalyzed by a mutant chitinase with lower hydrolyzing activity.