Improvement of the growth defect in salt- and ethanol-tolerant yeast mutagenized with error-prone DNA polymerization by using backcross cell fusion

Hayashi K; Yano S; Abe H; Fujita Y; Kishida M

Journal of Bioscience and Bioengineering, Vol.114, No.4, 476-478, 2012

DOI10.1016/j.jbiosc.2012.05.014 Export Citation

Improvement of the growth defect in salt- and ethanol-tolerant yeast mutagenized with error-prone DNA polymerization by using backcross cell fusion

Hayashi K, Yano S, Abe H, Fujita Y, Kishida M

Salt- and ethanol-tolerant mutants of Saccharomyces cerevisiae, isolated from the uracil-requiring mutant derived from Taiken No. 396 by proofreading-deficient DNA polymerization, showed less growth than their parent strain. The fusants, between these tolerant mutants and the lysine-requiring mutant from Taiken No. 396 obtained by the protoplast fusion, indicated improved growth. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Salt-tolerant yeast;Ethanol-tolerant yeast;Cell fusion;Backcross;Error-prone DNA polymerase;Saccharomyces cerevisiae