A small DNA rectangular parallelepiped block was prepared from a single stranded scaffold strand of 1152 nt long and 28 staple strands by DNA origami method. The scaffold strand, which was obtained by PCR using a phosphorylated primer followed by lambda exonuclease digestion, was folded into the designed structure. Subsequent assembly of the DNA block into higher order DNA nanostructures was achieved by slow annealing conditions in the presence of linker strands, giving both the tail-to-tail dimer and the tail-to-head one-dimensional oligomer with a choice of appropriate linker strands.