The aim of the present investigation was to optimize protocol for conversion of yellow sarson (Brassica campestris L.) subsp. Oleifera oil to biodiesel. The maximum yield of biodiesel was obtained at 75 degrees C in the presence of 1% NaOH (w/w oil) and methanol/oil molar ratio of 9:1. The quality parameters of biodiesel, such as specific gravity, color, kinematic viscosity, flash, cloud, and pour point, were in accordance with the American Society for Testing Materials (ASTM) D 6751.