Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: Color-based selection of colonies using the met30 strain

Tarutina MG; Dutova TA; Yezhova IE; Nishiuchi H; Sineoky SP

Journal of Bioscience and Bioengineering, Vol.114, No.6, 619-621, 2012

DOI10.1016/j.jbiosc.2012.07.011 Export Citation

Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: Color-based selection of colonies using the met30 strain

Tarutina MG, Dutova TA, Yezhova IE, Nishiuchi H, Sineoky SP

Glutathione overproducers were detected by examining the pigmentation intensity of Saccharomyces cerevisiae met30 yeast carrying wild-type alleles for ADE1 and ADE2. Highly pigmented colonies, phenocopies of the ade2 or ade1 mutants, were observed among yeast grown in minimal biotin-free medium with a high methionine content. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Visual selection of colonies;Glutathione;Yeast with red pigmentation;Saccharomyces cerevisiae;ade1 mutation;ade2 mutation