In this work, the hydrolysis reaction of 2-propyl-1,3-dioxane (2PD) prepared by the reactive extraction of 1,3-propanediol (1,3-PD) in dilute fermentation broth with butyraldehyde was studied experimentally. The equilibrium and reaction kinetics of the hydrolysis reaction were established catalyzed by cation exchange resin HD-8. After eliminating the influence of internal and external diffusion, the rate constants of positive and reverse reaction were obtained by fitting the experimental data to a macro-kinetics model. The results indicated that the reaction was an endothermal reaction. The results can help to design the hydrolysis reactor of 2PD.