사자발쑥 추출물의 피부 흡수 증진을 위한 에토좀 제형에 관한 연구

양현갑; 김혜진; 김해수; 박수남; Hyun Gab Yang; Hye Jin Kim; Hae Soo Kim; Soo Nam Park

Applied Chemistry for Engineering, Vol.24, No.2, 190-195, April, 2013

Export Citation

사자발쑥 추출물의 피부 흡수 증진을 위한 에토좀 제형에 관한 연구

Ethosome Formulation for Enhanced Transdermal Delivery of Artemisia princeps Pampanini Extracts

양현갑, 김혜진, 김해수, 박수남 ^†

서울과학기술대학교 정밀화학과, 나노바이오화장품연구실, 화장품종합기술연구소

Hyun Gab Yang, Hye Jin Kim, Hae Soo Kim, and Soo Nam Park^†

Department of Fine Chemistry, Nanobiocosmetic Laboratory and Cosmetic R&D Center, Seoul National University of Science and Technology, Seoul 139-743, Korea

E-mail:

초록

이전 연구에서 저자들은 사자발쑥 추출물의 항산화, 항노화 및 항균활성에 대한 결과를 보고한 바 있다. 본 연구에서는 사자발쑥 추출물의 피부 전달시스템으로 에토좀을 제조하고 입자크기, 포집효율 및 피부 투과를 평가하였다. 0.06% 사자발쑥 추출물의 에틸아세테이트 분획을 담지한 에토좀은 3주 동안 보다 더 안정하였고 일정한 입자크기를 유지하였다. 0.06% 에틸아세테이트 분획을 함유한 에토좀의 입자 크기는 287.05 ± 0.25 nm, 포집효율은 51.96 ± 0.01%였다. 피부 투과 실험 결과, 에토좀 제형은 일반 리포좀이나 20% 에탄올 용액에서 보다 큰 피부 투과능을 보여주었다.

In our previous studies, the antioxidant, anti-aging, and antibacterial activities of Artemisia princeps Pampnini (A. princeps Pamp.) extract were reported. In this study, ethosome formulations for the enhanced transdermal delivery of A. princeps Pamp. extract were prepared. The particle size, loading efficiency and skin permeation of them were evaluated. The ethosome loaded with 0.06% ethyl acetate fraction of A. princeps Pamp. extract was more stable and maintained the constant particle size for 3 weeks after being prepared. The particle size of ethosome containing 0.06% ethyl acetate fraction was 287.05 ± 0.25 nm and the loading efficiency was 51.96 ± 0.01%. The ethosome formulation exhibited the greater enhancement of skin permeation than of general liposome and 20% ethanol solution in skin permeability experiments.

Keywords:ethosomes;Artemisia princeps Pampanini;skin permeation studies;cosmetics

[References]

Yang HJ, Ahn YJ, Kim JH, Park SN, J. Soc. Cosmet.Scientists Korea., 34, 189 (2008)
Park SN, J. Korean Ind. Eng. Chem., 14(5), 657 (2003)
Chen Q, Fischer A, Reagan JD, Yan L, Ames BN, Proc. Natl. Sci. USA., 92, 4337 (1995)
Kim JE, Chae KY, Park SN, J. Soc. Cosmet. Scientists Korea., 37, 265 (2011)
Scharffetter-Kochanek K, Photoaging of the connective tissue of skin: Its prevention and therapy, In: Antioxidants in disease mechanisms and therapy, eds. H. Sies, Advances Pharmacology (1996)
Packer L, Utraviolet radiation (UVA, UVB) and skin antioxidants, In: Free radical damage and its control, eds. Rice-Evans CA, Burdon RH, Elsevier Science B.V. (1994)
Park SN, J. Soc. Cosmet. Scienctists Korea., 23, 75 (1997)
Thiele JJ, Barland CO, Ghadially R, Elias RN, Permeability and antioxidant Barriers in aged epidermis, Skin Aging (Gilchrest BA, Krutman J, eds), Springer-Verlag Berlin Heidelberg, Germany, 65 (2006)
Michaels AS, Chandrasekaran SK, Shaw JE, AlChE J., 21, 985 (1975)
Honeywell-Nguyen PL, Bouwstra JA, Drug Discovery Today: Technologies., 2, 67 (2005)
Jin BS, Han YM, Lee KH, Choi MJ, J. Korean Ind. Eng. Chem., 15(2), 205 (2004)
Elsayed MMA, Abdallah OY, Naggar VF, Khalafallah NM, Int. J. Pharm., 332, 1 (2007)
Touitou E, Dayan N, Bergelson L, Godin B, Eliaz M, J.Control. Release., 65, 403 (2000)
Gwak HJ, Jin BS, J. Korean Ind. Eng. Chem., 18(2), 130 (2007)
Dubey V, Mishra D, Dutta T, Nahar M, Saraf DK, Jain NK, J. Control. Release., 123, 148 (2007)
Dubey V, Mishra D, Jain NK, Eur. J. Pharm. Biopharm., 67, 398 (2007)
Adachi T, Takahashi H, Ohki K, Hatta I, Biophysical J., 68, 1850 (1995)
Bang MH, Cho JG, Song MC, Lee DY, Han MW, Chung HG, Jeong TS, Lee KT, Choi MS, Baek NI, J. Korean Soc. Appl. Biol. Chem., 51, 223 (2008)
Bang MH, Kim DH, Yoo JS, Lee DY, Song MC, Yang HJ, Jeong TS, Lee KT, Choi MS, Chung HG, Baek NI, J. Kor. Soc. Appl. Biol. Chem., 48, 418 (2005)
Yoo JS, Ahn EM, Bang MH, Song MC, Yang HJ, Kim DH, Lee DY, Chung HG, Jeong TS, Lee KT, Choi MS, Baek NI, Kor. J. Medicinal. Crop. Sci., 14, 273 (2006)
Chang SH, Jung EJ, Park YH, Lim DG, Ko NY, Choi WS, Her E, Kim SH, Choi KD, Bae JH, Kim SH, Kang CD, Han DJ, Kim SC, J. Pharm. Pharmacol., 61, 1043 (2009)
Junga UJ, Baek NI, Chung HG, Bang MH, Yoo JS, Jeong TS, Lee KT, Kang YJ, Lee MK, Kim HJ, Yeo JY, Choi MS, Food Chem. Toxicol., 45, 2022 (2007)
Yang HG, Kim HJ, Kim HS, Park SN, Korean J. Microbiol. Biotechnol., 40, 250 (2012)
Fang YP, Tsai YH, Wu PC, Huang YB, Int. J. Pharm., 356, 144 (2008)
Paolino D, Lucania G, Mardente D, Alhaique F, Fresta M, J. Control. Release., 106, 99 (2005)
Fang YP, Huang YB, Wu PC, Tsai YH, Eur. J.Pharm. Biopharm., 73, 391 (2009)

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[3] Chen Q, Fischer A, Reagan JD, Yan L, Ames BN, Proc. Natl. Sci. USA., 92, 4337 (1995)

[4] Kim JE, Chae KY, Park SN, J. Soc. Cosmet. Scientists Korea., 37, 265 (2011)

[5] Scharffetter-Kochanek K, Photoaging of the connective tissue of skin: Its prevention and therapy, In: Antioxidants in disease mechanisms and therapy, eds. H. Sies, Advances Pharmacology (1996)

[6] Packer L, Utraviolet radiation (UVA, UVB) and skin antioxidants, In: Free radical damage and its control, eds. Rice-Evans CA, Burdon RH, Elsevier Science B.V. (1994)

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[8] Thiele JJ, Barland CO, Ghadially R, Elias RN, Permeability and antioxidant Barriers in aged epidermis, Skin Aging (Gilchrest BA, Krutman J, eds), Springer-Verlag Berlin Heidelberg, Germany, 65 (2006)

[9] Michaels AS, Chandrasekaran SK, Shaw JE, AlChE J., 21, 985 (1975)

[10] Honeywell-Nguyen PL, Bouwstra JA, Drug Discovery Today: Technologies., 2, 67 (2005)

[11] Jin BS, Han YM, Lee KH, Choi MJ, J. Korean Ind. Eng. Chem., 15(2), 205 (2004)

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[18] Bang MH, Cho JG, Song MC, Lee DY, Han MW, Chung HG, Jeong TS, Lee KT, Choi MS, Baek NI, J. Korean Soc. Appl. Biol. Chem., 51, 223 (2008)

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[20] Yoo JS, Ahn EM, Bang MH, Song MC, Yang HJ, Kim DH, Lee DY, Chung HG, Jeong TS, Lee KT, Choi MS, Baek NI, Kor. J. Medicinal. Crop. Sci., 14, 273 (2006)

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[24] Fang YP, Tsai YH, Wu PC, Huang YB, Int. J. Pharm., 356, 144 (2008)

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