Here, we present a case study on a Wyoming well with known asphaltene deposition issues as a result of natural depletion. Field deposits and crude oil from the same well were collected for analysis. Compositional differences between field deposits, lab-generated capillary deposits, and C-7-precipitated asphaltenes were determined by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), and all three samples show similar trends in composition, displayed as plots of aromaticity versus carbon number. An enrichment of highly condensed aromatic molecules for the field deposit is detected with both ultrahigh resolution mass spectrometry and thermal cracking experiments and could predict asphaltene deposition FT-ICR mass spectral analysis of solvent extracted fractions suggest different deposition mechanisms for field deposits (slow deposition) compared to rapid precipitation in standard asphaltene preparation protocols that contain trapped maltenes.