Aims To in vitro evaluate the influence of the corn on the adsorption levels of aflatoxin B1 (AFB1) and zearalenone (ZEA) by yeast cell walls (YCWs). Methods and Results Two commercial YCWs were studied. The YCWs contain different percentages of polysaccharides. YCW1 and 2 contain 5 center dot 9 and 21% of mannans and 17 center dot 4 and 23% of -glucans, respectively. Each YCW was resuspended in pH 2 and pH 6 buffer solutions. Corn was used to study the matrix influence. An aliquot of 500l YCW suspension was added to each microtube containing 500l of 0 center dot 1, 0 center dot 25, 0 center dot 5, 1, 2 center dot 5 and 5gml1 AFB1 or 0 center dot 5, 5, 10, 20 and 50gml1 ZEA. Microtubes were kept with mechanical agitation at 37 degrees C for 30min and then centrifuged for 10min at 16873g and; the supernatants were quantified by high-pressure liquid chromatography. The amount of bound toxin was plotted as a function of the amount of added toxin according to mathematical expressions proposed by three theoretical models. Both YCWs were capable of adsorbing AFB1 and ZEA in amounts from 0 center dot 061 to 0 center dot 40 and from 0 center dot 10 and 0 center dot 26gg1, respectively. In the presence of the matrix, both adsorbents were not able to adsorb AFB1. However, they could adsorb ZEA at levels from 0 center dot 03 to 0 center dot 23gg1. Conclusions Both YCWs adsorbed ZEA in the presence of corn and also under simulated gastrointestinal pH conditions. These results suggest that the studied YCWs are potential candidates for ZEA adsorption. Significance and Impact of the Study Several in vitro assays have informed the ability of different substrates including yeast walls to adsorb AFB1 and ZEA; none of them have evaluated their ability to adsorb AFB1 and ZEA in the presence of the corn. The corn matrix can influence the adsorption phenomena of these mycotoxins.