This paper discusses the application of chronopotentiometry to study of membrane electroporation. The experiments were performed on bilayer lipid membranes formed using the painting technique. Phosphatidylcholine and cholesterol were chosen for the study because they fulfill essentials functions in living organisms. The studies have been carried out at various forming solution compositions and at various potassium ion concentrations in the electrolyte solution. Based on chronopotentiometric curves and mathematical equations, the membrane capacitance, resistance, and breakdown voltage as well as pore conductance and diameter were calculated. (C) 2013 The Electrochemical Society. [DOI: 10.1149/2.051303jes] All rights reserved.