Herbal drug loaded poly(D,L-lactide-co-glycolide) ultrafine fibers: Interaction with pathogenic bacteria

Hanan M. Al-Youssef; Musarat Amina; Shamshi Hassan; Touseef Amna; Jin Won Jeong; Ki-Taek Nam; Hak Yong Kim

Macromolecular Research, Vol.21, No.6, 589-598, June, 2013

DOI10.1007/s13233-013-1062-1 Export Citation

Herbal drug loaded poly(D,L-lactide-co-glycolide) ultrafine fibers: Interaction with pathogenic bacteria

Hanan M. Al-Youssef¹, Musarat Amina¹, Shamshi Hassan², Touseef Amna^{3, †}, Jin Won Jeong², Ki-Taek Nam², and Hak Yong Kim^{3, 2, †}

¹Department of Pharmacognosy, College of pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia
²Department of Organic Materials and Fiber Engineering, Chonbuk National University, Jeonbuk, 561-756, Korea
³Center for Healthcare Technology Development, Chonbuk National University, Jeonbuk, 561-756, Korea

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This study investigated for the first time the antimicrobial effect of methanolic extract of Grewia mollis Juss (mGM) against Staphylococcus aureus KCCM 11256 (Gram positive) and Escherichia coli KCCM 11234 (Gram negative). The minimum inhibitory concentration (MIC) values for each bacterial strain were recorded in a liquid medium. In addition, ultrafine poly(D,L-lactide-co-glycolide) (PLGA) fiber mats containing a methanolic crude extract (7.5 wt%) of Grewia mollis (mGM) were fabricated from the neat PLGA solution by electrospinning. The PLGA ultrafine fiber mat containing mGM were characterized by SEM, FE-SEM, Bio-TEM, TGA, and EDX analyses. Our findings indicate that the incorporation of mGM in the polymer media affected both the morphology and size of the mGM-loaded PLGA ultrafine fibers (UFs). SEM-EDX results confirmed well oriented ultrafine fibers with good incorporation of mGM. Results of the structure characterization indicated that the incorporated mGM interacted strongly with the PLGA matrix and the mechanical properties were effectively improved. Moreover, mGM loaded PLGA ultrafine fiber mats were able to inhibit the growth of the bacterial strains, which indicate that mGM blended PLGA fibers could be used as a potential effective antimicrobial agent for many biomedical applications including wound dressing.

Keywords:pathogenic bacteria;antibacterial mechanism;G. mollis;PLGA;antibacterial drug

[References]

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Cushnie TP, Lamb AJ, J. Antimicrob. Chemoter., 26, 343 (2005)
Zhang L, Kong Y, Wu D, Zhang H, Wu J, Chen J, Ding J, Hu L, Jiang H, Shen X, Protein Sci., 17, 1971 (2008)

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[2] Newman DJ, Cragg GM, J. Nat. Prod., 70, 461 (2007)

[3] Pai MR, Acharya LD, Udupa N, J. Ethnopharmacol., 90, 99 (2004)

[4] Baris O, Gulluce M, Sahin F, Ozer H, Kilic H, Ozkan H, Sokmen M, Ozbek T, Turk. J. Biol., 30, 65 (2006)

[5] Sill TJ, Von HAR, Biomaterials, 29, 1989 (2008)

[6] Cui WG, Zhou Y, Chang J, Sci. Technol. Adv. Mater., 11, 1 (2010)

[7] Puppi D, Piras AM, Detta N, Dinucci D, Chiellini F, Acta Biomater., 6, 1258 (2010)

[8] Martins E, Christiana I, Olobayo K, Afr. J. Pharm. Pharmacol., 2, 1 (2008)

[9] Muazu J, Musa H, Musa KY, J. Pharm. Sci. Technol., 1, 74 (2009)

[10] Onwuliri FC, Mawak JD, Wonang DL, Onwuliri EA, Int. J. Nat. Appl. Sci., 2, 225 (2006)

[11] Schulz M, Strack D, Weissenbock G, Markham KR, Dellamonica G, Chopin J, Phytochemical, 24, 343 (1985)

[12] Matsuda H, Cai H, Kubo M, Tosa H, Inuma M, Biol. Pharm. Bull., 18, 462 (1995)

[13] Dapkevicius A, Beek TA, Lelyveld GP, Veldhuizen A, Groot A, Linssen JPH, Venskutonis R, J. Nat. Prod., 65, 892 (2002)

[14] Lee MH, Son YK, Han YN, Arch. Pharm. Res., 25, 842 (2002)

[15] EI-Negoumy SI, Mansour RMA, Grasas Aceites (Seville), 40, 87 (1989)

[16] Kausano G, Idoji M, Sogoh Y, Shibano M, Kusano A, Iwashita T, Chem. Pharm. Bull., 42, 1106 (1994)

[17] Banskota AH, Tezuka Y, Tran KQ, Tanaka K, Saiki I, Kadota S, J. Nat. Prod., 63, 57 (2000)

[18] Do JC, Son KH, Kang SS, Korean. J. Pharmacogn., 19, 170 (1988)

[19] Park SW, Yook CS, Lee HK, Korean. J. Pharmacogn., 25, 328 (1994)

[20] Xu X, Yang L, Xu X, Wang X, Chen X, Liang Q, Zeng J, Jing X, J. Control. Release, 108, 33 (2005)

[21] Verreck G, Chun I, Rosenblatt J, Peeters J, Van AD, Mensch J, Noppe M, Brewster ME, J. Control. Release, 92, 349 (2003)

[22] Elijah IN, Barbara RC, Carbohydr. Polym., 84, 446 (2011)

[23] Jose MV, Thomas V, Dean DR, Nyairo E, Polymer, 50(15), 3778 (2009)

[24] Cushnie TP, Lamb AJ, J. Antimicrob. Chemoter., 26, 343 (2005)

[25] Zhang L, Kong Y, Wu D, Zhang H, Wu J, Chen J, Ding J, Hu L, Jiang H, Shen X, Protein Sci., 17, 1971 (2008)