화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.21, No.6, 660-668, June, 2013
DOI10.1007/s13233-013-1076-8  Export Citation
Intracellular polysaccharide and its antioxidant activity by Pleurotus citrinopileatus SM-01
Su-Qian Wu, Shang-Long Gao, Hong-Hong Liu, Xin-Yi Sun, Long Hao, Le Jia†, Li-Fei Pang1, Shou-Hua Jia1, †, and Meng-Shi Jia2
  • College of Life Science, Shandong Agricultural University, Taian, Shandong, 271018, P. R. China
  • 1College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong, 271018, P. R. China
  • 2The Second High School of Taian, Taian, Shandong, 271000, P. R. China
E-mail:,
The extraction parameters of intracellular polysaccharide (IPS) from Pleurotus citrinopileatus SM-01 mycelia were optimized, and the in vitro and in vivo antioxidant activities of IPS were investigated. The optimum conditions of IPS extraction were predicted to be an ultrasonic treatment time of 664.09 s, precipitation time of 23.03 h and pH 7.36, and IPS yield was estimated at 16.13%. The in vitro inhibition effects of IPS at a dosage of 5 g/L on the superoxide anion, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals were 73.96±4.62%, 69.2±4.37%, and 50.75±4.39%, respectively, which were 72.56±5.08%, 22.83±1.94%, and 43.93±3.26% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of IPS was 0.9±0.07, 69.81±5.24% higher than that of BHT. The activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) and alanine aminotransferase (ALT) in mice blood were 241.38±23.19, 454.95±42.39, 60.32±5.16, and 32.39±2.54 U/mL, respectively, and the malonaldehyde (MDA) level was 9.54±0.72 nmol/mL. The results provided a reference for the large-scale extraction of IPS by P. citrinopileatus SM-01 in industrial fermentation, suggesting that the IPS can be used as a potential antioxidant, which enhances adaptive immune responses.
Keywords:Pleurotus citrinopileatus SM-01;intracellular polysaccharide;antioxidant activity;in vitro;in vivo
  1. Kalmi E, Azbar N, Kalyoncu F, Can. J. Microbiol., 54, 366 (2008)
  2. Wang JC, Hu SH, Liang ZC, Yeh CJ, Appl. Microbiol. Biotechnol., 67(6), 759 (2005)
  3. Confortin FG, Marchetto R, Bettin F, Camassola M, Salvador M, Dillon AJ, J. Ind. Microbiol. Biotechnol., 35, 1149 (2008)
  4. Jia L, Hu SZ, Xu M, Ann. Microbiol., 57, 389 (2007)
  5. Zhang M, Zhu L, Cui SW, Wang Q, Zhou T, Shen H, Int. J. Biol. Macromol., 48, 5 (2011)
  6. Cho EJ, Oh JY, Chang HY, Yun JW, J. Biotechnol., 127, 129 (2006)
  7. Cui JD, Zhang BZ, Lett. Appl. Microbiol., 52, 123 (2011)
  8. Meng FY, Zhou B, Lin RS, Jia L, Liu XN, Deng P, Fan KM, Wang GY, Wang L, Zhang JJ, Bioresour. Technol., 101(12), 4564 (2010)
  9. Hao LM, Xing XH, Li Z, Zhang JC, Sun JX, Jia SR, Qiao CS, Wu TY, Appl. Biochem. Biotechnol., 160(2), 621 (2010)
  10. Lung MY, Huang PC, Lett. Appl. Microbiol., 50, 198 (2010)
  11. Chen W, Zhao Z, Chen SF, Li YQ, Bioresour. Technol., 99(8), 3187 (2008)
  12. Baskar G, Sathya SR, Int. J. Med. Mushrooms, 13, 455 (2011)
  13. Liu XN, Zhou B, Lin RS, Jia L, Deng P, Fan KM, Wang GY, Wang L, Zhang JJ, Int. J. Biol. Macromol., 47, 116 (2010)
  14. Jin WJ, Lu XX, J. Anhui Agricultural Science, 39, 16545 (2011)
  15. Li ZP, Wu P, J. Anhui Science Technology University, 23, 19 (2009)
  16. Huang ZW, Hu JX, Peng ZM, Zhou HS, Cui YH, J. Yangzhou University, 31, 90 (2010)
  17. Zhang ZX, Zhang HH, Ma HL, Food Research Development, 29, 17 (2008)
  18. Chen W, Wang WP, Zhang HS, Huang Q, Carbohydr. Polym., 87, 614 (2012)
  19. Chaplin MF, Kennedy JF, Carbohydrate Analysis, A Practical Approach, IRL Press Ltd., New York, 1994.
  20. Plackett RL, Burman JP, Biometrika, 33, 305 (1946)
  21. Stewar RC, Beewley JD, Plant Physiol., 65, 245 (1980)
  22. Liu X, Zhao M, Food Fermentation Industry, 32, 151 (2006)
  23. Winterbourn CC, Sutton HC, Arch. Biochem. Biophys., 235, 116 (1984)
  24. Oyaizu M, Jpn. J. Nutr., 44, 307 (1986)
  25. Flohe L, Guunzler WA, Methods Enzymol., 105, 114 (1984)
  26. Bayer WF, Fridovich JL, Anal. Biochem., 161, 559 (1987)
  27. Schlorff EC, Husain K, Somani SM, Alcohol, 2, 97 (1999)
  28. Wu GH, Wang YQ, He DL, He P, Huang ZL, Natural Product Research and Development, 21, 334 (2009)
  29. Zhao SJ, Shi GA, Dong XC, Experiment Instruction of Plant Physiology, China Agricultural Press, Beijing, 2002.
  30. An JS, Wang D, Ma SQ, Du M, Zhao XL, Teng LR, Wang ZZ, Food Research and Development, 29, 11 (2008)
  31. Fu JN, Liu XH, Cai FD, Li SF, Lu H, Transactions of the Chinese Society for Agricultural Machinery, 6, 98 (2008).
  32. Ma C, Dong XP, Zhu BW, J. Dalin Institute of Light Industry, 24, 195 (2005)
  33. Xu H, Sun LP, Zhang B, Zhao DQ, China Brewing, 168, 44 (2007)
  34. Shi DF, Zhou Y, Li X, Chen L, Q. J. Fungal Research, 6, 226 (2008)
  35. Slater TF, Biochem. J., 222, 1 (1984)
  36. Kohen R, Nyska A, Toxicol. Pathol., 30, 620 (2002)
  37. Athukorala Y, Kim KN, Jeon YJ, Food Chem. Toxicol., 44, 1065 (2006)
  38. Yang F, Wang XF, Weng L, Ge QM, Chen SS, Food Science, 31, 76 (2010)
  39. Jayakumar T, Thomas PA, Sheu JR, Geraldine P, Food Res. Int., 44, 851 (2011)
  40. Xie LY, Zhang Y, Peng WH, Gan BC, Food Science, 32, 276 (2011)
  41. Shimada K, Fujikawa K, Yahara K, Nakamura T, J. Agric. Food Chem., 40, 945 (1992)
  42. Reis FS, Martins A, Barros L, Ferreira IC, Food Chem. Toxicol., 50, 1201 (2012)
  43. Lee YL, Jian SY, Lian PY, Mau JL, J. Food Compos. Anal., 21, 116 (2008)
  44. Tseng YH, Yang JH, Mau JL, Food Chem., 107, 732 (2008)
  45. Kim IG, Jung IL, Oh TJ, Kim KC, Shim HW, Biotechnol. Lett., 24(16), 1299 (2002)
  46. Fan KM, Meng GY, Zhou B, Deng P, Liu XN, Jia L, Wang GY, Wang L, Zhang JJ, J. Appl. Polym. Sci., 120(3), 1744 (2011)
  47. Zhang H, Mu ZB, Xu LM, Xu GF, Liu M, Shan AS, Lipids, 44, 643 (2009)
  48. Chance B, Sies H, Boveris A, Physiol. Rev., 59, 527 (1979)
  49. Guo SB, Chinese J. Sports Medicine, 9, 167 (1990)
  50. Niki E, Yoshida Y, Saito Y, Noguchi N, Biochem. Biophys. Res. Commun., 338(1), 668 (2005)