Chitosan effects on glass matrices evaluated by biomaterial. MAS-NMR and biological investigations

Hassane Oudadesse; Eric Wers; Xuang Vuong Bui; Claire Roiland; Bruno Bureau; Imane Akhiyat; Amany Mostafa; Hassan Chaair; Hicham Benhayoune; Joel Fauré; Pascal Pellen-Mussi

Korean Journal of Chemical Engineering, Vol.30, No.9, 1775-1783, September, 2013

DOI10.1007/s11814-013-0104-x Export Citation

Chitosan effects on glass matrices evaluated by biomaterial. MAS-NMR and biological investigations

Hassane Oudadesse^{1, †}, Eric Wers¹, Xuang Vuong Bui¹, Claire Roiland¹, Bruno Bureau¹, Imane Akhiyat^{1, 2}, Amany Mostafa^{1, 3}, Hassan Chaair⁴, Hicham Benhayoune⁵, Joel Fauré⁵, and Pascal Pellen-Mussi¹

¹SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042 Rennes, France
²Faculté des Sciences et Techniques de Mohammedia (FSTM), Université Hassan II, Maroc, France
³Biomaterilas Department, National Research Centre (NRC), 12622 Dokki, Cairo, Egypt
⁴Faculté des Sciences et Techniques de Mohammedia (FSTM), Université Hassan II, Maroc, Morocco
⁵LISM-URCA, 21 rue Clément Ader, BP 138, 51685, Reims Cedex 02, France

E-mail:

Bioactive glass 46S6 and biodegradable therapeutic polymer (Chitosan: CH) have been elaborated to form 46S6-CH composite by freeze-drying process. The kinetics of chemical reactivity and bioactivity at the surface were investigated by using physicochemical techniques, particularly solid-state MAS-NMR. Immortalized cell line used to construct multicellular spheroids was employed as three-dimensional (3D) cell cultures for in vitro studies. Obtained results showed a novel structure of the composite; the chemical treatment (ultrasound, magnetic stirring, freeze drying process and lyophilization) led the bioactive glass particles to be loaded in the chitosan-based materials. 29Si and 31P MAS-NMR results showed the emergence of two new species, QSi3(OH) and QSi4, which are characteristic of the vitreous network dissolution in simulated body fluid (SBF). MAS-NMR also confirmed the formation of amorphous calcium phosphate (ACP) at the surface of the initial 46S6-CH. Three-dimensional (3D) cell cultures highlighted the effect of chitosan, where the cell viability reached up to 78% in 46S6-CH composite and up to 67% in 46S6. The association of (CH) and bioactive glass (BG) matrix promotes a highly significant bioactivity, demonstrating surface bone formation and satisfactory behavior in biological environment.

Keywords:Bioactive Glass;Chitosan;Composite;Bioactivity;Hydroxyapatite;31P MAS NMR;29Si MAS NMR;Immortalized Cell;3D Cells Viability

[References]

Hench LL, Splinter RJ, Allen WC, Greenlee TK, J.Biomed. Mater. Res., 5, 117 (1971)
Hench LL, J. Sci. Mater: Mater. Med., 17, 967 (2006)
Rich J, Jaakkola T, Tirri T, Narhi T, Yli-Urpo A, Seppala C, J. Biomater., 23, 2143 (2002)
Walsh D, Furuzono T, Tanaka A, J. Biomater., 22, 1205 (2001)
Devin J, Attawia M, Laurencin C, J. Sci. Biomater. Polym. Edn., 7, 661 (1996)
Leonor IB, Baran ET, Kawashita M, Reis RL, Kokubo T, Nakamura T, Acta Biomater., 4, 1349 (2008)
Thein-Han WW, Misra RDK, Acta Biomater., 5, 1182 (2008)
Brandenberg G, Leibrock LG, Shuman R, Malette WG, Neurosur., 15, 9 (1984)
Muzzarelli RA, Tanfani F, Emanuelli M, Pace DP, Chiumzzi E, Res. Carbohyd., 126, 225 (1984)
Hirano S, Yagi Y, Res. Carbohyd., 8, 103 (1980)
Jiang L, Li Y, Wang X, Zhang L, Wen J, Gong M, Carbohyd.Poly., 74, 680 (2008)
Zhao L, Chang J, J. Sci. Mater: Mater. in Med., 15, 625 (2004)
Joel Bumgardner D et al., US Patent, 0254007 A1 (2007)
Mathew P, Binulal NS, Soumya S, Nair SV, Furuike T, Tamura H. Jayakumar R, Carbohyd. Poly., 79, 281 (2009)
Boyan BD, et al., US Patent, 5,492,697 (1996)
Dietrich E, Oudadesse H, Lucas-Girot A, Mami M, J. Biomed.Mater. Res. A., 88, 1087 (2008)
Bui XV, Oudadesse H, Gal YL, Merdrignac-Conanec O, Cathelineau G, Korean J. Chem. Eng., 29(2), 215 (2012)
Bui XV, PhD, Elaboration de biomateriaux verres-substances actives (Zoledronate-Chitosan), Order Number 4421, University of Rennes 1, France, 171, 21 November 2011.
Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro T, J.Biomed. Mater. Res., 24, 721 (1990)
Massiot D, Fayon F, Capron M, King I, Le Calve S, Alonso B, Durand JO, Bujoli B, Gan Z, Hoaston G, Magn. Reson.Chem., 40, 70 (2002)
Lockyer MWG, Holland D, Dupree R, J. Non. Cryst. Solids., 188, 207 (1995)
Elgayar I, Aliev AE, Boccaccini AR, Hill RG, J. Non. Cryst.Solids., 351, 173 (2005)
Angelopoulou A, Montouillout V, Massiot D, Kordas G, J.Non. Cryst. Solids., 354, 333 (2008)
Hinedi ZR, Goldberg S, Chang AC, Yesinowski JP, J. Colloid Interface Sci., 152, 141 (1992)
Belton PS, Harris RK, Wilkes PJ, J. Phys. Chem. Solids., 49, 21 (1988)
Aue WP, Roufosse AH, Glimcher MJ, Griffin RG, Biochem., 23, 6110 (1984)
Lin KSK, Tseng TH, Mou Y, Hsu YC, Yang CM, Chan JCC, Chem. Mater., 17, 4493 (2005)
Philips NG, YHLo AY, Isabel IB, Ana G, Daniel A, Baltzar S, Jekabs G, Maria VR, Mattias E, J. Phys. Chem. C., 114, 9345 (2010)
Diaz A, Lopez T, Manjarrez J, Basaldella E, Martinez-Blanes JM, Odriozola JA, Acta Biomater., 2, 173 (2006)
Oudadesse H, Dietrich E, Bui XV, Le Gal Y, Pellen P, Cathelineau G, Appl. Surf. Sci., 257(20), 8587 (2011)
Machenzie KJD, Smith ME, Multinuclear solid-state NMR of inorganic materials, Cambridge: Pergamon Mater Series (2002)
Oestrike R, Yang WH, Kirkpatrick RJ, Hervig RL, Navrotsky A, Montez B, Geochem. Cosmochim Acta., 51, 2199 (1987)
Oudadesse H, USTV, October 2011, Rennes, France (2011)
Anderson OH, Karlsson KH, J. Non. Cryst. Solids., 129, 145 (1991)
Alno N, Jegoux F, Pellen-Mussi P, Tricot S, Oudadesse H, Cathelineau G, De Mello G, Biomed GJ, Mater. Res. A., DOI:10.1002/jbm.a.32818.
Dietrich E, PhD, Syntheses et etudes physic-chimiques de verres bioactifs denses et poreux, Order Number 3767, University of Rennes 1, France, 205, 17 October 2008.

[Referenced By]

[1] Hench LL, Splinter RJ, Allen WC, Greenlee TK, J.Biomed. Mater. Res., 5, 117 (1971)

[2] Hench LL, J. Sci. Mater: Mater. Med., 17, 967 (2006)

[3] Rich J, Jaakkola T, Tirri T, Narhi T, Yli-Urpo A, Seppala C, J. Biomater., 23, 2143 (2002)

[4] Walsh D, Furuzono T, Tanaka A, J. Biomater., 22, 1205 (2001)

[5] Devin J, Attawia M, Laurencin C, J. Sci. Biomater. Polym. Edn., 7, 661 (1996)

[6] Leonor IB, Baran ET, Kawashita M, Reis RL, Kokubo T, Nakamura T, Acta Biomater., 4, 1349 (2008)

[7] Thein-Han WW, Misra RDK, Acta Biomater., 5, 1182 (2008)

[8] Brandenberg G, Leibrock LG, Shuman R, Malette WG, Neurosur., 15, 9 (1984)

[9] Muzzarelli RA, Tanfani F, Emanuelli M, Pace DP, Chiumzzi E, Res. Carbohyd., 126, 225 (1984)

[10] Hirano S, Yagi Y, Res. Carbohyd., 8, 103 (1980)

[11] Jiang L, Li Y, Wang X, Zhang L, Wen J, Gong M, Carbohyd.Poly., 74, 680 (2008)

[12] Zhao L, Chang J, J. Sci. Mater: Mater. in Med., 15, 625 (2004)

[13] Joel Bumgardner D et al., US Patent, 0254007 A1 (2007)

[14] Mathew P, Binulal NS, Soumya S, Nair SV, Furuike T, Tamura H. Jayakumar R, Carbohyd. Poly., 79, 281 (2009)

[15] Boyan BD, et al., US Patent, 5,492,697 (1996)

[16] Dietrich E, Oudadesse H, Lucas-Girot A, Mami M, J. Biomed.Mater. Res. A., 88, 1087 (2008)

[17] Bui XV, Oudadesse H, Gal YL, Merdrignac-Conanec O, Cathelineau G, Korean J. Chem. Eng., 29(2), 215 (2012)

[18] Bui XV, PhD, Elaboration de biomateriaux verres-substances actives (Zoledronate-Chitosan), Order Number 4421, University of Rennes 1, France, 171, 21 November 2011.

[19] Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro T, J.Biomed. Mater. Res., 24, 721 (1990)

[20] Massiot D, Fayon F, Capron M, King I, Le Calve S, Alonso B, Durand JO, Bujoli B, Gan Z, Hoaston G, Magn. Reson.Chem., 40, 70 (2002)

[21] Lockyer MWG, Holland D, Dupree R, J. Non. Cryst. Solids., 188, 207 (1995)

[22] Elgayar I, Aliev AE, Boccaccini AR, Hill RG, J. Non. Cryst.Solids., 351, 173 (2005)

[23] Angelopoulou A, Montouillout V, Massiot D, Kordas G, J.Non. Cryst. Solids., 354, 333 (2008)

[24] Hinedi ZR, Goldberg S, Chang AC, Yesinowski JP, J. Colloid Interface Sci., 152, 141 (1992)

[25] Belton PS, Harris RK, Wilkes PJ, J. Phys. Chem. Solids., 49, 21 (1988)

[26] Aue WP, Roufosse AH, Glimcher MJ, Griffin RG, Biochem., 23, 6110 (1984)

[27] Lin KSK, Tseng TH, Mou Y, Hsu YC, Yang CM, Chan JCC, Chem. Mater., 17, 4493 (2005)

[28] Philips NG, YHLo AY, Isabel IB, Ana G, Daniel A, Baltzar S, Jekabs G, Maria VR, Mattias E, J. Phys. Chem. C., 114, 9345 (2010)

[29] Diaz A, Lopez T, Manjarrez J, Basaldella E, Martinez-Blanes JM, Odriozola JA, Acta Biomater., 2, 173 (2006)

[30] Oudadesse H, Dietrich E, Bui XV, Le Gal Y, Pellen P, Cathelineau G, Appl. Surf. Sci., 257(20), 8587 (2011)

[31] Machenzie KJD, Smith ME, Multinuclear solid-state NMR of inorganic materials, Cambridge: Pergamon Mater Series (2002)

[32] Oestrike R, Yang WH, Kirkpatrick RJ, Hervig RL, Navrotsky A, Montez B, Geochem. Cosmochim Acta., 51, 2199 (1987)

[33] Oudadesse H, USTV, October 2011, Rennes, France (2011)

[34] Anderson OH, Karlsson KH, J. Non. Cryst. Solids., 129, 145 (1991)

[35] Alno N, Jegoux F, Pellen-Mussi P, Tricot S, Oudadesse H, Cathelineau G, De Mello G, Biomed GJ, Mater. Res. A., DOI:10.1002/jbm.a.32818.

[36] Dietrich E, PhD, Syntheses et etudes physic-chimiques de verres bioactifs denses et poreux, Order Number 3767, University of Rennes 1, France, 205, 17 October 2008.