화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.30, No.9, 1784-1789, September, 2013
DOI10.1007/s11814-013-0108-6  Export Citation
Anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages
Mi-Ra Chang, Woong Hee Lee1, Won Jong Rhee2, Tai Hyun Park3, and Eun Jeong Kim†
  • Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, Doryong-dong, Yuseong-gu, Daejeon 305-340, Korea
  • 1Department of Dermatology, Seoul National University College of Medicine, 28, Yeongeon-dong, Jongno-gu, Seoul 110-744, Korea
  • 2Division of Bioengineering, Incheon National University, 119, Academy-ro, Yeonsu-gu, Incheon 406-772, Korea
  • 3School of Chemical and Biological Engineering, Bio-MAX Institute, Seoul National University, 1, Gwanak-ro, Gwanak-gu, Seoul 151-744, Korea
E-mail:
Macrophages participate in several inflammatory pathologies such as sepsis and arthritis. We investigated the effect of silkworm hemolymph (SH) on the LPS-induced pro-inflammatory macrophages. SH inhibits LPSinduced nitric oxide (NO) production in RAW 264.7 cells and murine peritoneal macrophages. The decreased NO was reflected as a decreased amount of inducible nitric oxide synthase (iNOS) mRNA and protein. It was also found that SH inhibited pro-inflammatory cytokines, IL-1β, IL-6, and TNF-α production. To elucidate the mechanism by which SH inhibits NO production and iNOS expression, we investigated that SH suppressed IκB phosphorylation, which leads to the activation of NF-κB followed by degradation of IκB. This observation suggests that SH is a potential therapeutic modulator for inflammation-associated disorders.
Keywords:Macrophages;Silkworm Hemolymph;Nitric Oxide (NO);Inducible Nitric Oxide Synthase (iNOS);Pro-inflammatory Cytokines
  1. Janeway CA, Medzhitov R, Annu. Rev. Immunol., 20, 197 (2002)
  2. Raetz CR, Whitfield C, Annu. Rev. Biochem., 71, 635 (2002)
  3. Petros A, Bennett D, Vallance P, Lancet., 338, 1557 (1991)
  4. Cohen J, Nature., 420, 885 (2002)
  5. ter Steege JC, van de Ven MW, Forget PP, Brouckaert P, Buurman WA, Cytokine., 10, 115 (1998)
  6. Cheon H, Yu SJ, Yoo DH, Chae IJ, Song GG, Sohn J, Clin. Exp. Immunol., 127, 547 (2002)
  7. Xie QW, Kashiwabara Y, Nathan C, J. Biol. Chem., 269, 4705 (1994)
  8. Sha WC, Liou HC, Tuomanen EI, Baltimore D, Cell., 80, 321 (1995)
  9. Ha SH, Park TH, Kim SE, Biotechnol. Technol., 10, 401 (1996)
  10. Rhee WJ, Kim EJ, Park TH, Biotechnol. Prog., 15(6), 1028 (1999)
  11. Ha SH, Park TH, Biotechnol. Lett., 19(11), 1087 (1997)
  12. Rhee WJ, Park TH, Biochem. Biophys. Res. Commun., 271, 186 (2002)
  13. Choi SS, Rhee WJ, Park TH, Biotechnol. Prog., 18(4), 874 (2002)
  14. Rhee WJ, Kim EJ, Park TH, Biophys. Res. Commun., 295, 779 (2002)
  15. Kim EJ, Rhee WJ, Park TH, Biochem. Biophys. Res. Commun., 285(2), 224 (2001)
  16. Kim EJ, Rhee WJ, Park TH, Biotechnol. Prog., 20(1), 324 (2004)
  17. Kim EJ, Park HJ, Park TH, Biochem. Biophys. Res. Commun., 308(3), 523 (2003)
  18. Kim EJ, Park TH, Biotechnol. Bioprocess Eng., 8, 76 (2003)
  19. Choi SS, Rhee WJ, Kim EJ, Park TH, Biotechnol. Bioeng., 95(3), 459 (2006)
  20. Wang Z, Park JH, Park HH, Tan WS, Park TH, Biotechnol. Bioeng., 108(7), 1634 (2011)
  21. Park JH, Wang Z, Jeong HJ, Park HH, Kim BG, Tan WS, Choi SS, Park TH, Appl. Microbiol. Biotechnol., 96(3), 671 (2012)
  22. Joosten CE, Park TH, Shuler ML, Biotechnol. Bioeng., 83(6), 695 (2003)
  23. Choi SS, Rhee WJ, Park TH, Biotechnol. Bioeng., 91(7), 793 (2005)
  24. Rhee WJ, Lee EH, Park TH, Biotechnol. Bioprocess Eng., 14, 645 (2009)
  25. Rhee WJ, Lee EH, Park JH, Lee JE, Park TH, Biotechnol. Prog., 23(6), 1441 (2007)
  26. D’Acquisto F, May MJ, Ghosh S, Mol. Interv., 2, 22 (2002)
  27. Starnes HF, Pearce MK, Tewari A, Yim JH, Zou JC, Abrams JS, J. Immunol., 145, 4185 (1990)
  28. Rollins BJ, Mol. Med. Today., 2, 198 (1996)
  29. Van Kaer L, Immunol. Cell Biol., 82, 315 (2004)
  30. Ghosh S, May MJ, Kopp EB, Annu. Rev. Immunol., 16, 225 (1998)
  31. Xaus J, Comalada M, Valledor AF, Lioberas J, Lopez-Soriano F, Argiles JM, Bogdan C, Celada A, Blood., 15, 3823 (2000)
  32. Koizumi N, Morozumi A, Imamura M, Tanaka E, Iwahana H, Sato R, Eur. J. Biochem., 248, 217 (1997)
  33. Koizumi N, Imamura M, Kadotani T, Yaoi K, Iwahana H, Sato R, Febs Lett., 443, 139 (1999)
  34. Kamimura M, Nakahara Y, Kanamori Y, Tsuzuki S, Hayakawa Y, Kiuchi M, Biochem. Biophys. Res. Commun., 286(1), 67 (2001)
  35. Ishii K, Hamamoto H, Kamimura M, Nakamura Y, Noda H, Imamura K, Mita K, Sekimizu K, J. Biol. Chem., 285, 28635 (2010)