A comparative study on production of middle chain diacylglycerol through enzymatic esterification and glycerolysis

Dan-Jing Hu; Jun-Ming Chen; Yong-Mei Xia

Journal of Industrial and Engineering Chemistry, Vol.19, No.5, 1457-1463, September, 2013

DOI10.1016/j.jiec.2013.01.009 Export Citation

A comparative study on production of middle chain diacylglycerol through enzymatic esterification and glycerolysis

Dan-Jing Hu, Jun-Ming Chen, and Yong-Mei Xia^†

State Key Laboratory of Food Science and Technology, School of Chemical and Materials Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China

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A comparative study on lipase catalyzed production of middle chain diacylglycerol was conducted with esterification of glycerol and glycerolysis of triacylglycerol, respectively, in which high diacylglycerol yield and high acyl donor conversion are two desired goals. The esterification provided much higher acyl donor conversion than glycerolysis (with the conversion of 94.42% and 74.21%, respectively). The esterification in packed bed reactor produced more diacylglycerol than that in batch reactor (with relative content in the product of 77.26% and 45.45%, respectively). Mass transfer is one of the limitations during the procedure. Microwave irradiation provides higher reaction rate and selectivity in glycerolysis.

Keywords:Diacylglycerol;Esterification;Glycerolysis;Batch reactor;Packed bed reactor;Enzyme

[References]

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[Referenced By]

[1] Jackson MA, Appell M, Berhow MA, Blackburn JA, Rheiner SN, Journal of the American Oil Chemists Society., 89, 713 (2012)

[2] Pelicand J, Maes M, Charlier D, Aujoulat I, Archives De Pediatrie., 19, 585 (2012)

[3] Erion DM, Shulman GI, Nature Medicine., 16, 400 (2010)

[4] Reddy PS, Sudarsanam P, Raju G, Reddy BM, J. Ind. Eng. Chem., 18(2), 648 (2012)

[5] Duan ZQ, Du W, Liu DH, Journal of Biotechnology., 159, 44 (2012)

[6] Chen HC, Kuo CH, Tsai WC, Chung YL, Chiang WD, Chang CMJ, Liu YC, Shieh CJ, Journal of the American Oil Chemists Society., 89, 231 (2012)

[7] Matos LMC, Leal ICR, de Souza ROMA, Journal of Molecular Catalysis BEnzymatic., 72, 36 (2011)

[8] Wang Y, Zhao MM, Ou SY, Song KK, Han X, European Journal of Lipid Science and Technology., 111, 652 (2009)

[9] Wang Y, Zhao MM, Ou SY, Song KK, Journal of Food Lipids., 16, 113 (2009)

[10] Wang Y, Zhao M, Ou S, Xie L, Tang S, Journal of Molecular Catalysis B-Enzymatic., 56, 165 (2009)

[11] Nissinen MJ, Vuoristo M, Gylling H, Miettinen TA, Lipids., 42, 603 (2007)

[12] Bogalhos P, Fregolente L, Pinto GMF, Wolf-Maciel MR, Maciel R, Appl. Biochem. Biotechnol., 160(7), 1879 (2010)

[13] Liu N, Wang Y, Zhao QZ, Cui C, Fu M, Zhao MN, Food Chemistry., 134, 301 (2012)

[14] Zhong NJ, Li L, Xu XB, Cheong LZ, Zhao XH, Li B, Food Chemistry., 122, 228 (2010)

[15] Liu N, Wang Y, Zhao QZ, Zhang QL, Zhao MM, European Journal of Lipid Science and Technology., 113, 973 (2011)

[16] Rosu R, Yasui M, Iwasaki Y, Yamane T, Journal of the American Oil Chemists Society., 76, 839 (1999)

[17] Tai HP, Brunner G, Journal of Supercritical Fluids., 59, 87 (2011)

[18] Wan HD, Hu XY, Seo YG, Xia YM, J. Ind. Eng. Chem., 18(5), 1564 (2012)

[19] Yeoh CM, Choong TSY, Abdullah LC, Yunus R, Siew WL, European Journal of Lipid Science and Technology., 111, 599 (2009)

[20] Teixeira ARS, Santos JLC, Crespo JG, Ind. Eng. Chem. Res., 51(15), 5443 (2012)

[21] Watanabe T, Sugiura M, Sato M, Yamada N, Nakanishi K, Process Biochemistry., 40, 637 (2005)

[22] Voll F, Kruger RL, de Castilhos F, Cardozo L, Cabral V, Ninow J, Corazza ML, Biochemical Engineering Journal., 56, 107 (2011)

[23] Fang Y, Sun SY, Xia YM, Journal of Molecular Catalysis B-Enzymatic., 55, 6 (2008)

[24] Laane C, Boeren S, Vos K, Veeger C, Biotechnology and Bioengineering., 30, 81 (1987)

[25] Fang Y, Huang W, Xia YM, Process Biochemistry., 43, 306 (2008)