In this work, treatment of oily wastewaters with commercial polyacrylonitrile (PAN) ultrafiltration (UF) membranes was investigated. In order to do these experiments, the outlet wastewater of the API (American Petroleum Institute) unit of Tehran refinery, is used as the feed. The purpose of this paper was to predict the permeation flux and fouling resistance, by applying artificial neural networks (ANNs), and then to optimize the operating conditions in separation of oil from industrial oily wastewaters, including trans-membrane pressure (TMP), cross-flow velocity (CFV), feed temperature and pH, so that a maximum permeation flux accompanied by a minimum fouling resistance, was acquired by applying genetic algorithm as a powerful soft computing technique. The experimental input data, including TMP, CFV, feed temperature and pH, permeation flux and fouling resistance as outputs, were used to create ANN models. This fact that there is an excellent agreement between the experimental data and the predicted values was shown by the modeling results. Eventually, by multi-objective optimization, using genetic algorithm (GA), an optimization tool was created to predict the optimum operating parameters for desired permeation flux (i.e. maximum flux) and fouling resistance (i.e. minimum fouling) behavior. The accuracy of the model is confirmed by the comparison between the predicted and experimental data. (C) 2012 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.