One of the major causes of diesel engine maintenance problems is water contamination in fuels. This paper discusses fabrication, characterization, and testing of non-woven glass fiber mats augmented with an electrospun layer of poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) fibers for the separation of secondary water dispersions from ultralow sulfur diesel. The PVDF-HFP fibers had an average fiber diameter of 334 nm. All PVDF-HFP layers had water contact angles greater than 150 degrees with relatively low hysteresis values, making them superhydrophobic. Water separation experiments showed that glass fiber media augmented with electrospun PVDF-HFP fiber mats significantly improved water separation, with water removal efficiencies reaching 99%, as compared to glass fiber media without the PVDF-HFP layer.