Two endochitinases from Lactococcus lactis and Bacillus halodurans were expressed in E. coli, and the protein production process was performed at a bioreactor scale. The effect of engineering variables such as aeration and agitation on the performance of the biological process was determined; maximum levels of activity were achieved at an aeration rate of 0.33 vvm and an agitation rate of 300 rpm when using isopropyl-beta-D-thiogalactopyranoside (IPTG) as an inducer. The use of lactose as an inducer in cultures of L. lactis generated maximum levels of chitinolytic activity (2.25 U/L) in just 3 h of cultivation, a level much higher than for other reported strains. The production process was kinetically analyzed using unstructured models, and the Luedeking and Piret equation was used to characterize the enzyme produced.