Aims: The study set out to sample Euro10 banknotes for the presence of coagulase-positive staphylococci (CPS) such as Staphylococcus aureus (S.aureus) and coagulase-negative staphylococci (CoNS) in Southern Ireland, to assess the levels of antibiotic resistance among those isolated, and determine the persistence of S.aureus on Euro10 banknotes and Euro2 coins. Methods and Results: We report that 97% of Euro10 banknotes screened (n=155) harboured multiple species of staphylococci. From the generated bank of strains, a total of 150 representative staphylococci isolates were used for further study, 71 were CPS and 79 were CoNS. Of these, we found that 62% of the staphylococci demonstrated resistance to at least one of the first-line antibiotics (5211% of CPS isolates and 7671% of the CoNS isolates). Resistance to multiple antibiotics was seen in 3118% of the resistant isolates. In relation to persistence studies, S.aureus was shown to remain viable on euro banknotes and coins for significant periods (on average, 1933days on Euro10 banknotes and 1667days on Euro2 coins) as determined using bioluminescence. Conclusions: We advocate the expansion of antibiotic surveillance programs, with a view to tracking/monitoring antibiotic resistance dissemination among environmental contaminants. Additionally, we propose that cashless transactions' should be encouraged in high-risk environments such as hospitals and healthcare settings, as well as stricter infection controls. Significance and Impact of the Study: Although it is accepted that circulating currency has the potential to harbour disease-causing pathogens, studies investigating prevalence and persistence of such pathogens on euro currency are virtually nonexistent. In an attempt to rectify this, we examined the prevalence of staphylococci on Euro10 banknotes in Ireland and reported relatively high levels of antibiotic resistance among the isolates. Furthermore, we have established the persistence of S.aureus on euro currency for the first time.