To incorporate microorganisms and to preserve their integrity, new matrices of poly(N-acryloylglycine) have been designed under appropriate conditions. To understand the interactions between the microorganisms and the organic part of the matrices, different conetworks of poly(N-acryloylglycine) have been synthesized and characterized. Copolymerization with two crosslinkers was performed with different compositions. The thermal and swelling properties of conetworks are specifically controlled and compared. These investigations show that the swelling ratio of these materials is compatible with the incorporation of biomolecules in these matrices. They successfully permit Pseudomonas species 1625 bacteria incorporation. The biological activity of bacteria is also preserved, allowing the use of these materials for innovative biological applications. (C) 2013 Wiley Periodicals, Inc.