Chemically defined media and auxotrophy of the prolific L-lactic acid producer Lactococcus lactis IO-1

Machii M; Watanabe S; Zendo T; Chibazakura T; Sonomoto K; Shimizu-Kadota M; Yoshikawa H

Journal of Bioscience and Bioengineering, Vol.115, No.5, 481-484, 2013

DOI10.1016/j.jbiosc.2012.11.024 Export Citation

Chemically defined media and auxotrophy of the prolific L-lactic acid producer Lactococcus lactis IO-1

Machii M, Watanabe S, Zendo T, Chibazakura T, Sonomoto K, Shimizu-Kadota M, Yoshikawa H

Two chemically defined media, CDM-1G and CDM-1X, that use glucose and xylose as carbon sources, respectively, were prepared for Lactococcus lactis strain IO-1. The maximal cell density at 600 nm in CDM-1G exceeded 2. Omission growth experiments indicated that IO-1 is auxotrophic for 2 vitamins and 6 amino acids. (c) 2012, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Growth factor;Nutritional requirement;Maximal growth rate;Biomass;Xylose utilization;Polylactic acid;Lactic acid bacteria