화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Applied Chemistry for Engineering, Vol.24, No.5, 558-561, October, 2013
Export Citation
Phosphate Binding Protein이 세포질에 발현된 재조합 박테리아를 이용한 인 제거
Phosphate Removal Using Recombinant Bacteria with Cytoplasmic Phosphate Binding Protein
최석순†, 하정협1, 차형준2
  • 세명대학교 바이오환경공학과
  • 1포항공과대학교 환경공학부
  • 2포항공과대학교 화학공학과
Suk Soon Choi†, Jeong Hyub Ha1, and Hyung Joon Cha2
  • Department of Biological and Environmental Engineering, Semyung University, Jecheon 390-711, Korea
  • 1School of Environmental Science and Technology, Pohang University of Science and Technology, Pohang 790-784, Korea
  • 2Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea
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초록
본 연구에서는 인 결합 단백질(phosphate-binding protein)이 세포질(cytoplasm)에서 발현되는 재조합 대장균(Escherichia coli)을 제조하였으며, 이를 이용하여 수중에 함유된 인의 제거에 대하여 고찰하였다. 이 균주를 6 h 동안 배양하여 1.0, 1.5, 2.0 mg/L의 인을 처리하였을 때, 각각 90, 49, 41%의 제거효율을 도출하였으며, 우수한 인 제거 능력(specific phosphate removal)을 나타내었다. 또한, 세포의 Optical density를 2.5, 5.0으로 조절하여 운전하였을 때, 인의 제거효율이 향상됨을 알 수 있었으며, 2.0 mg/L 인을 80%까지 제거시킬 수 있었다. 이 연구를 통하여 얻어진 새로운 생물기술은 수계의 부영양화 문제를 해결하는데 효과적으로 사용될 수 있을 것이다.
In the present work, we constructed a recombinant Escherichia coli with cytoplasmic-expressed phosphate-binding protein (PBP) and investigated its phosphate removal in water phase. When the recombinant bacteria were cultured for 6 h to treat phosphate, the removal efficiencies were 90, 49, and 41% for the treatment of 1.0, 1.5, and 2.0 mg/L phosphate, respectively, indicating good specific phosphate removal of our developed system. Also, cell densities of 2.5 and 5.0 Optical density resulted in high phosphate removal efficiencies and ∼80% of 2.0 mg/L phosphate was efficiently removed. A novel biotechnology developed in this study could be effectively employed for resolving eutrophication problem in water body.
Keywords:cytoplasmic phosphate-binding protein;biological phosphate removal;removal efficiency of phosphate
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