알로인이 함유된 창상치료제용 PVA/CMC 하이드로젤의 개발

김진; 이창문; 김동운; 이기영; Jin Kim; Chang Moon Lee; Dong-Woon Kim; Ki-Young Lee

Polymer(Korea), Vol.37, No.6, 802-808, November, 2013

Export Citation

알로인이 함유된 창상치료제용 PVA/CMC 하이드로젤의 개발

Development of Aloin Loaded PVA/CMC Hydrogel for Wound Healing

김진, 이창문¹, 김동운², 이기영 ^{3, †}

전남대학교 신화학소재공학과
¹전남대학교 의공학과
²광양보건대학교 임상병리과
³전남대학교 응용화학공학부 & 촉매연구소

Jin Kim, Chang Moon Lee¹, Dong-Woon Kim², and Ki-Young Lee^{3, †}

Department of Advanced Chemicals and Engineering, Chonnam National University, Gwangju 500-757, Korea
¹Department of Biomedical and Engineering, Chonnam National University, Gwangju 500-757, Korea
²Department of Clinical Pathology, Gwangyang Health College, Gwangyang 545-703, Korea
³Korea Faculty of Applied Chemical Engineering & The Research Institute for Catalysis, Chonnam National University, Gwangju 500-757, Korea

E-mail:

초록

알로인이 첨가된 하이드로젤을 제조하여 창상치유의 효과를 관찰하였다. Poly(vinyl alcohol)(PVA)과 carboxyl methyl cellulose(CMC)를 동결-융해 방법으로 결합시킨 하이드로젤을 제조하였다. 젤의 특성과 약물의 방출, in vivo 창상치유 실험 및 병리학적 평가를 진행하였다. 쥐 등에 만든 상처의 치유 실험은 알로인이 첨가된 PVA/CMC(5:5) 하이드로젤 그룹과 대조군 그룹의 알로인이 함유된 카보머934 젤 혹은 순수 카보머934 젤로 처치한 그룹보다 CMC의 수분과 알로인의 피토케미칼 활성 때문에 빠른 치유력을 확인할 수 있었다. 결론적으로 알로인이 첨가된 창상용 드레싱으로 5% PVA, 5% CMC 그리고 0.125% 알로인의 조성은 상처치료에 효과적이며 창상용 드레싱으로 활용을 기대할 수 있다.

The purpose of this study was to develop an aloin-loaded wound dressing with an enhanced healing effect. The cross-linked hydrogel films were prepared with poly(vinyl alcohol) (PVA) and carboxyl methyl cellulose (CMC) using a freeze-thawing (F-T) method. Their gel properties, release of drug, in vivo wound healing effect and histopathology were then evaluated. In the wound healing test, this aloin loaded PVA/CMC hydrogel showed faster healing of the wound made in rat dorsum than the aloin carbomer 934 gel or the control (carbomer 934 gel) due to phytochemical activity of aloin and moisture of CMC. In conclusion, the aloin-loaded wound dressing composed of 5% PVA, 5% CMC and 0.125% aloin is a potential wound dressing with enhanced wound healing effect.

Keywords:aloin;poly(vinyl alcohol) (PVA);carboxyl methyl cellulose (CMC);wound heal.

[References]

Chithra P, Sajithlal GB, Chandrakasan G, J., 59, 179 (1998)
Czubryt MP, Fibrogenesis & Tissue Repair, 5, 19 (2012)
Marchese A, Shito G, Int. J. Antimicrob. Ag., 16, 25 (2001)
Reynolds T, Dweck AC, J. Ethnopharmacol., 68, 3 (1999)
Park MY, Kwon HJ, Sung MK, Biosci. Biotechnol. Biochem., 73, 828 (2009)
Chen W, Wyk BEV, Vermaak I, Viljoen AM, Phytochem. Lett., 5, 1 (2012)
Femenia A, Sanchez ES, Simal S, Rossello C, Carbohydr.Polym., 39, 109 (1999)
Getie M, Mariam TG, Reitz R, Neubert RH, Pharmazie., 57, 320 (2002)
Shetty S, Udupa S, Udupa L, Evid. Based Complement.Alternat. Med., 5, 95 (2008)
Barrantes E, Guinea M, Life Sci., 72, 843 (2003)
Jo SY, Lim YM, Youn MH, Gwon HJ, Park JS, Nho YC, Shin H, Polym.(Korea), 33(6), 551 (2009)
Lee JH, Lim SJ, Oh DH, Ku SK, Li DX, Yong CS, Choi HG, Arch. Pharm. Res., 33, 1083 (2010)
Pines E, Rins W, Macromolecules., 6, 888 (1973)
Huang MH, Yang MC, Int. J. Pharm., 346, 38 (2008)
Yagi A, Egusa T, Arase M, Tanabe M, Tsuji H, Planta Med., 63, 18 (1997)
Jettanacheawchankit S, Sasithanasate S, Sangvanich P, J.Pharmacol. Sci., 109, 525 (2009)
Singer AJ, Clark RA, N. Engl. J. Med., 341, 738 (1999)
Hosgood G, “Wound Repair and Specific Tissue to Injury”, in Textbook of Small Animal Surgery, 3rd edition, D. Slatter, Editor, Saunders, 2, 66 (2003)

[Referenced By]

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[1] Chithra P, Sajithlal GB, Chandrakasan G, J., 59, 179 (1998)

[2] Czubryt MP, Fibrogenesis & Tissue Repair, 5, 19 (2012)

[3] Marchese A, Shito G, Int. J. Antimicrob. Ag., 16, 25 (2001)

[4] Reynolds T, Dweck AC, J. Ethnopharmacol., 68, 3 (1999)

[5] Park MY, Kwon HJ, Sung MK, Biosci. Biotechnol. Biochem., 73, 828 (2009)

[6] Chen W, Wyk BEV, Vermaak I, Viljoen AM, Phytochem. Lett., 5, 1 (2012)

[7] Femenia A, Sanchez ES, Simal S, Rossello C, Carbohydr.Polym., 39, 109 (1999)

[8] Getie M, Mariam TG, Reitz R, Neubert RH, Pharmazie., 57, 320 (2002)

[9] Shetty S, Udupa S, Udupa L, Evid. Based Complement.Alternat. Med., 5, 95 (2008)

[10] Barrantes E, Guinea M, Life Sci., 72, 843 (2003)

[11] Jo SY, Lim YM, Youn MH, Gwon HJ, Park JS, Nho YC, Shin H, Polym.(Korea), 33(6), 551 (2009)

[12] Lee JH, Lim SJ, Oh DH, Ku SK, Li DX, Yong CS, Choi HG, Arch. Pharm. Res., 33, 1083 (2010)

[13] Pines E, Rins W, Macromolecules., 6, 888 (1973)

[14] Huang MH, Yang MC, Int. J. Pharm., 346, 38 (2008)

[15] Yagi A, Egusa T, Arase M, Tanabe M, Tsuji H, Planta Med., 63, 18 (1997)

[16] Jettanacheawchankit S, Sasithanasate S, Sangvanich P, J.Pharmacol. Sci., 109, 525 (2009)

[17] Singer AJ, Clark RA, N. Engl. J. Med., 341, 738 (1999)

[18] Hosgood G, “Wound Repair and Specific Tissue to Injury”, in Textbook of Small Animal Surgery, 3rd edition, D. Slatter, Editor, Saunders, 2, 66 (2003)