화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Chemical Engineering Research, Vol.51, No.6, 709-715, December, 2013
Export Citation
맥주 폐 효모액의 당화 및 에탄올 발효능
Saccharification and Fermentation Capability of the Waste from Beer Fermentation Broth
강민경, 김민아1, 유보완, 박중곤†
  • 경북대학교 화학공학과, 702-701 대구시 북구 대학로 80
  • 1계명대학교 화학공학과, 704-701 대구시 달서구 달구벌대로 1095
MinKyung Kang, Minah Kim1, Bowan Yu, and Joong Kon Park†
  • Department of Chemical Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 702-701, Korea
  • 1Department of Chemical Engineering, Keimyung University, 1095 Dalgubeoldae-ro, Dalseo-gu, Daegu 704-701, Korea
E-mail:
초록
맥주 폐 효모액(waste from beer fermentation broth, WBFB)은 바이오 에탄올 생산을 위한 우수하고 저렴한 원료이다. 본 연구에서는 바이오 에탄올 생산을 위해 WBFB의 당화능과 발효능을 확인하는 실험을 진행하였다. 당화능은 온도를 30, 40, 50, 60, 70 ℃로 다르게 하여 실험했는데 온도가 올라감에 따라 당화능은 증가하였고 4시간 후 60 ℃와 70 ℃에서 많은 양의 glucose가 생산되었다. WBFB와 chemically defined media (CDM) 혼합물에서는 어떠한 미생물의 첨가 없이도 발효가 되어 에탄올이 생산되었다. 동시당화발효능을 30, 40, 50, 60 ℃의 다양한 온도에서 실험해본 결과 30 ℃에서 에탄올이 가장 많이 생산되었다. 또 이 실험은 WBFB, starch 용액 그리고 CDM을 이용하여 수행하였는데 WBFB에 있는 당화 효소와 효모가 어떠한 추가적 미생물 첨가 없이 당화와 발효를 가능케 하는 요인이었다.
The waste from beer fermentation broth (WBFB) has been found an excellent and inexpensive resource for bioethanol production. We tried to evaluate the saccharification and fermentation capabilities of WBFB to confirm its effectiveness for bioethanol production. The saccharification potentials of the WBFB were evaluated at various temperatures (30, 40, 50, 60and 70 ℃). It was found that the saccharification capabilities increased with temperature and highest reached maximum at 60 ℃ and 70 ℃ after 4h. Ethanol production from a mixture of WBFB and chemically defined media (CDM) without addition of any microbial species confirmed the fermentation capabilities of WBFB. Simultaneous saccharification and fermentation were performed using WBFB, starch solution and CDM as culturing media. The maximum yield of bioethanol production was obtained at 30 ℃. The saccharifying enzymes and the yeast cells present in WBFB were essential factors for the production of bioethanol from WBFB without any additional enzymes or microbial cells.
Keywords:Bio-ethanol;Thermotolerant Yeast;SSF;WBFB
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