Applied Chemistry for Engineering, Vol.24, No.6, 639-644, December, 2013
두충 추출물을 담지한 에토좀의 특성 및 경피 전달
Characterization and Transdermal Delivery of Ethosomes Loaded with Eucommia ulmoides Extract
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초록
본 연구에서는 화장품 소재로서 항염, 항노화 효능이 있는 두충 추출물을 담지한 에토좀을 제조하였고, 이들 에토좀이 안정한지를 확인하기 위하여 4주 동안 입자크기, 물리적 특성 및 포집효율을 측정하였다. 0.01∼0.05% 두충 추출물을 담지한 에토좀은 4주 동안 침전이나 분리 현상이 나타나지 않았다. 이들 에토좀의 평균 입자 크기는 134.6 nm였고, 단분산 형태를 나타내었다. 0.05% 두충 추출물을 함유한 에토좀의 입자 크기는 151.15 nm였으며, 포집 효율은 81.79% 로 에토좀 중 가장 큰 포집효율을 나타내었다. 0.05% 두충 추출물을 담지한 에토좀을 이용하여 피부 투과 실험을 수행하였다. 두충 추출물의 총 피부 투과량은 에토좀(77.16%) > 리포좀(62.80%) > 에탄올 용액(41.59%) 순으로 나타났다. 결과적으로 0.05% 두충 추출물을 담지한 에토좀은 피부 전달에 효과적이며, 식물 추출물을 함유하는 화장품 제형으로서 사용 가능성이 있음을 시사한다.
In this study, ethosomes loaded with Eucommia ulmoides extracts that have anti-inflammatory and anti-aging activities on the skin were prepared as cosmetic materials. The particle size, physical property, and entrapment efficiency of ethosomes to identify their stabilities during 4 weeks were measured. Ethosomes containing 0.01∼0.05% E. ulmoides extracts did not show either precipitation or separation. The mean particle size of these ethosomes was 136.4 nm, and monodisperse. The particle size of ethosome containing 0.05% E. ulmoides extracts was 151.15 nm and the entrapment efficiency was 81.79%. Therefore, in vitro skin permeation experiment was performed with ethosome containing 0.05% E. ulmoides extracts that showed the high loading efficiency and stability during 4 weeks. The order of skin permeation of E. ulmoides extracts was ethosome (77.16%) > liposome (62.80%) > ethanolic solutoin (41.59%). These results suggest that ethosomes containing 0.05% E. ulmoides extracts are effective for the skin permeation and may be used as a cosmetic formulation containing plant extracts.
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