Cell adhesion and surface chemistry of biodegradable aliphatic polyesters: Discovery of particularly low cell adhesion behavior on poly(3-[RS]-hydroxybutyrate)

Chan Woo Lee; Bong Keun Song; Jonggeon Jegal; Yoshiharu Kimura

Macromolecular Research, Vol.21, No.12, 1305-1313, December, 2013

DOI10.1007/s13233-013-1181-8 Export Citation

Cell adhesion and surface chemistry of biodegradable aliphatic polyesters: Discovery of particularly low cell adhesion behavior on poly(3-[RS]-hydroxybutyrate)

Chan Woo Lee^†, Bong Keun Song¹, Jonggeon Jegal¹, and Yoshiharu Kimura²

Department of Innovative Industrial Technology, Hoseo University, Asan, Chungnam, 336-851, Korea
¹Division of Convergence Chemistry, Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, Daejeon, 305-343, Korea
²Department of Biomolecular Engineering, Kyoto Institute of Technology Matsugasaki, Kyoto, 606-8585, Japan

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Histocompatibility of biodegradable aliphatic polyesters was examined by cell adhesion and cell proliferation tests by using fibroblast 3T3-L1 cells. It was found out that the cell adhesion decreased in the order of polystyrene (PS)>poly(L-lactide) (PLLA)>poly(3-[R]-hydroxybutyrate/valerate) ([R]-PHB/HV)>poly(butylene succinateco-lactide) (PBSL)>poly(3-[RS]-hydroxybutyrate) ([RS]-PHB). Particularly poor cell adhesion was shown for [RS]-PHB even after fibronectin had been pre-adsorbed, and cell proliferation and extension were not observed on [RS]-PHB. These different behaviors in cell adhesion could not be well explained by the differences in wettability and roughness of the film surfaces of these polymers. ATR-FTIR analysis of these films revealed that the surface concentration of methyl groups is significantly higher in the [RS]-PHB film than the other films. It was therefore considered that the poor cell adhesion of [RS]-PHB is attributable to the structural change of the protein adsorbed on the methyl-accumulated surface.

Keywords:biodegradable aliphatic polyester;cell adhesion;protein adsorption;surface structure

[References]

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Bhat VD, Truskey GA, Reichert WM, J. Biomed. Mater. Res., 41, 377 (1998)
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[9] Abe Y, Tsuchida H, Koubunshi to saiboumaku no sougosayou, Kyouritu Syuppan, 1998.

[10] Ikada Y, Biomaterial nyumon, 1994.

[11] Zhao K, Deng Y, Chen JC, Chen GQ, Biomaterials, 24, 1041 (2003)

[12] Chen GQ, Wu Q, Biomaterials, 26, 6565 (2005)

[13] Iyou zairyou no kagaku, Nihon kagakukai, 1978.

[14] Pierschbacher MD, Ruoslahti E, Nature, 309, 30 (1984)

[15] Bhat VD, Truskey GA, Reichert WM, J. Biomed. Mater. Res., 41, 377 (1998)

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[20] Smith L, Doyle C, Gregonis DE, Andrade JD, J. Appl. Polym. Sci., 26, 1269 (1982)

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[25] Tamada Y, Ikada Y, J. Colloid Interface Sci., 155, 334 (1993)

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