화학공학소재연구정보센터
Applied Biochemistry and Biotechnology, Vol.171, No.3, 771-785, 2013
Bioethanol Production from Ipomoea Carnea Biomass Using a Potential Hybrid Yeast Strain
The paper deals with the exploitation of Ipomoea carnea as a feedstock for the production of bioethanol. Dilute acid pretreatment under optimum conditions (3 %H2SO4, 120 A degrees C for 45 min) produced 17.68 g L-1 sugars along with 1.02 g L-1 phenolics and 1.13 g L-1 furans. A combination of overliming and activated charcoal adsorption facilitated the removal of 91.9 % furans and 94.7 % phenolics from acid hydrolysate. The pretreated biomass was further treated with a mixture of sodium sulphite and sodium chlorite and, a maximum lignin removal of 81.6 % was achieved. The enzymatic saccharification of delignified biomass resulted in 79.4 % saccharification with a corresponding sugar yield of 753.21 mg g(-1). Equal volume of enzymatic hydrolysate and acid hydrolysate were mixed and used for fermentation with a hybrid yeast strain RPRT90. Fermentation of mixed detoxified hydrolysate at 30 A degrees C for 28 h produced ethanol with a yield of 0.461 g g(-1). A comparable ethanol yield (0.414 g g(-1)) was achieved using a mixture of enzymatic hydrolysate and undetoxified acid hydrolysate. Thus, I. carnea biomass has been demonstrated to be a potential feedstock for bioethanol production, and the use of hybrid yeast may pave the way to produce bioethanol from this biomass.