Production of endoxylanase and beta-xylosidase by Aspergillus niger in solid state fermentation (SSF), submerged fermentation (SmF), and liquid surface fermentation (LSF) was studied. The production of endoxylanase and beta-xylanase by LSF resulted in the highest enzyme activity among the 3 fermentation modes. The maximum endoxylanase and beta-xylosidase activity in LSF was about 4.7 and 8.5 times that in SSF, respectively. The production of biomass and biomass yield in LSF was about 1.5 times higher than that in SmF. LSF was limited in the production of enzyme and biomass when the liquid film thickness is relatively high or is too low. The appropriate liquid level for LSF was found to be 0.5-1 mm.