We present the analysis of the 100 A 2SIGMA-100 X 2PI3/2 band of N2O+ observed by fast ion beam laser spectroscopy. The obtained spectrum allowed us to interpret the Fermi interaction effects in the X 2PI ground state. The two Fermi parameters W1 and W2 were determined for the first time. The obtained values are in good agreement with those calculated theoretically and those obtained for other isoelectronic linear triatomic molecules.