Quinine, quinidine, tert-butyl carbomylated quinine and tert-butyl carbomylated quinidine are proposed as new chiral selectors in the construction of enantioselective, potentiometric membrane electrodes (EPMEs). A matrix based on carbon paste is used for the design of the electrodes. Electrodes revealed near-Nernstian values for the concentration range of the analytes ((S) and (R)-dinitrobenzoyl leucine (DNB-Leu)) situated in low magnitude order, with low limits of detection (lower than 10(-7) mol/L). Quinine and quinide based EPMEs proved to be enantioselective for the (S)-DNB-Leu, while the tert-butyl carbomylated quinine based EPME was enantioselective for (R)-DNB-Leu. The tert-butyl carbamoyl quinidine based electrode was enantioselective only for specific ratios between the (S) and (R)- DNB-Leu enantiomers. (C) 2013 The Electrochemical Society. All rights reserved.