Polydiacetylenes have received intense attention on account of their well-established chromic alterations that are detectable often by the naked eye, making them ideal for a variety of applications such as biosensory materials. These polymers have been fabricated in a variety of materials platforms including 3D crystals, 2D monolayers, and 0D spherical vesicles; however, 1D morphologies that might be useful for directional energy migration are less common. This article describes the development and current research efforts of protein-based 1D nanowire-like supramolecular assemblies with embedded polydiacetylenes.