화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.22, No.3, 272-278, March, 2014
DOI10.1007/s13233-014-2036-7  Export Citation
Radiolabeled Chitosan Hydrogel Containing VEGF Enhances Angiogenesis in a Rodent Model of Acute Myocardial Infarction
Dae-Weung Kim1, 2, Chang Guhn Kim1, 3, Nam-Ho Kim4, Sam Youn Lee5, Moon Young Lee6, Eul Sig Choi6, Soon-Ah Park1, 3, Chang-Moon Lee7, Hyosook Hwang8, Seok Tae Lim8, Myung-Hee Sohn8, and Hwan-Jeong Jeong8, †
  • 1Department of Nuclear Medicine and Institute of Wonkwang Medical Science, Wonkwang University School of Medicine, Jeonbuk, 570-749, Korea
  • 2Research Unit of Molecular Imaging Agent (RUMIA), Wonkwang University School of Medicine, Jeonbuk, 570-749, Korea
  • 3, Korea
  • 4Department of Cardiovascular Medicine and Regional Cardiocerebrovascular Center, Wonkwang University Hospital, Jeonbuk, 570-749, Korea
  • 5Department of Cardiothoracic Surgery, Wonkwang University School of Medicine, Jeonbuk, 570-749, Korea
  • 6Department of Physiology, Wonkwang University School of Medicine, Jeonbuk, 570-749, Korea
  • 7Department of Biomedical Engineering, Chonnam National University, Jeonnam, 550-250, Korea
  • 8Department of Nuclear Medicine, Biomedical Research Institute, Institute for Medical Sciences, Cyclotron Research Center, Molecular Imaging & Therapeutic Medicine Research Center, Chonbuk National University Medical School and Hospital, Jeonbuk, 561-712, Korea
E-mail:
Vascular endothelial growth factor (VEGF) plays a pivotal role in angiogenesis in an infarcted myocardium. The purpose of the current investigation was to validate whether a radiolabeled VEGF delivery system can be effectively monitored in vivo, and to ascertain whether the growth factor induces an angiogenic effect to facilitate recovery from infarct conditions in rodent myocardial infarction (MI) models. Rat MI models were divided into three groups, one with left anterior descending coronary artery (LAD) ligation with no injection (the control group), one with LAD ligation and I-131 VEGF injection (the VEGF group), and one with LAD ligation and I-131 VEGF-loaded chitosan injection (the VIC group). On day 7 after injection, autoradiography imaging was performed, followed by semiquantitative and histopathologic analyses. In semi-quantitative analysis, the mean anterior-to-inferior wall ratio of the VIC group was significantly higher than those of the control and VEGF groups (p<0.05). Histopathologic experiments revealed a marked increase in microvascular density in the VIC group compared to those of the control and VEGF groups (p<0.05). Intramyocardially injected VIC was not only effectively monitored in vivo, but also stimulated therapeutic angiogenesis in the infarcted myocardium. Our findings support that the developed VIC is a novel theranostic tool to improve myocardial perfusion following myocardial infarction.
Keywords:myocardial infarction;vascular endothelial growth factor (VEGF);angiogenesis;chitosan hydrogel;theranosis.
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