효소를 이용한 부분 가수분해에 의한 isoquercitrin의 합성에 관한 연구

이슬비; 정대원; Seul Bi Lee; Dae-Won Chung

Applied Chemistry for Engineering, Vol.25, No.2, 152-156, April, 2014

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효소를 이용한 부분 가수분해에 의한 isoquercitrin의 합성에 관한 연구

Study on the Synthesis of Isoquercitrin by Enzymatic Partial Hydrolysis

이슬비, 정대원 ^†

수원대학교 공과대학 신소재공학과

Seul Bi Lee, and Dae-Won Chung^†

Department of Polymer Engineering, College of Engineering, Suwon University, Suwon 400-600, Korea

E-mail:

초록

Isoquercitrin (IQ)은 폴리페놀의 일종인 quercetin (QU)의 3번 위치에 glucose가 하나 결합해 있는 배당체로서, 천연에는 미량만이 존재하고 있다. 최근 QU의 2배당체인 rutin (RU)을 기질로 사용하여 효소에 의한 부분 가수분해에 의해 IQ를 합성할 수 있다는 것이 알려졌다. 본 논문에서는, PecinexTM 계열의 복합 효소를 사용하여 RU의 선택적 가수분해에 의해 IQ를 합성하는 반응에서 최적 효소를 선정하고, 효소량 및 반응물의 농도 등의 변수에 따른 반응성을 검토하였다. 그 결과, 50 ℃에서 1%의 반응물 농도로 RU 1 g 대비 8 mL의 Ultra Clear를 사용하는 것이 반응 속도 및 IQ의 선택성 측면에서 최적인 것으로 나타났다.

Isoquercitrin (IQ), quercetin monoglycoside, is classified as a polyphenol, and a minute quantity of IQ is known to be present in several plants. Recently, it was reported that IQ can be prepared by the partial enzymatic hydrolysis of quercetin diglycoside (rutin, RU). In this paper, the effects of enzyme types, enzyme amounts, and substrate concentrations on the reactivity were investigated using a series of PecinexTM multi-enzymes. The reaction, when a 8 ml of Ultra Clear to 1 g of RU was applied with the substrate concentration of 1% at 50 ℃, was found to be optimum, based on the reaction rate and the selectivity to IQ.

Keywords:isoquercitrin;quercetin;rutin;enzymatic hydrolysis

[References]

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Jeong EJ, Cha YJ, J. Life Sci., 22, 1665 (2012)
Hyun MR, Lee YS, Park YH, Kor. J. Hort. Sci. Technol., 29, 68 (2011)
Cheigh CI, Yoo SY, Chung MS, Kor. J. Food & Nutr., 24, 458 (2011)
Lee HJ, Park SN, J. Soc.Cosmet. Sci. Korea, 37, 143 (2011)
Li R, Yuan C, Dong C, Shuang S, Choi MMF, N. Schmied. Arch. Pharmacol., 383, 437 (2011)
Rogerio AP, Kanashiro A, Fontanari C, da Silva EV, Lucisano-Valim YM, Soares EG, Faccioli LH, Inflamm. Res., 56, 402 (2007)
Gasparotto Junior A , Gasparotto FM, Lourenco ELB, Crestani S, Stefanello MEA, Salvador MJ, da Silva-Santos JE, Marques MCA, Kassuya CAL, J. Ethnopharmacol., 134, 363 (2011)
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Amado NG, Cerqueira DM, Menezes FS, Mendes da Silva JF, Neto Vivaldo M, Abreu JG, Anti Canc. Drugs, 20, 543 (2009)
Williams BL, Wender SH, Arch.Biochem. Biophys., 43, 319 (1953)
Wang J, Lu D, Zhao H, Jiang B, Wang J, Ling X, Chai H, Ouyang P, J. Serb. Chem. Soc., 75, 875 (2010)
Mauludin R, Muller RH, Keck CM, Int. J. Pharm., 370, 202 (2009)
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Chebil L, Humeau C, Anthoni J, Dehez F, Engasser JM, Ghoul M, J. Chem. Eng. Data, 52(5), 1552 (2007)
You HJ, Ahn HJ, Ji GE, Agric.Food Chem., 58, 10886 (2010)
Weignerova L, Marhol P, Gerstorferova D, Kren V, Bioresource Tech., 115, 222 (2012)
HB Lee, EK Kim, SJ Park, SG Bang, TG Kim, DW Chung, J. Agric. Food Chem., 58, 4808 (2010)
DW Chung, SB Lee, J. Sci. Food Agric., 93, 362 (2013)
HB Lee, EK Kim, SJ Park, SG Bang, TG Kim, DW Chung, J. Sci. Food Agric., 91, 2315 (2011)
Lee SB, Chung DW, Appl. Chem. Eng., 23(4), 394 (2012)
Lee MH, Huh D, Jo DJ, Lee GD, Yoon SR, J. Korean Soc. Food Sci. Nutr., 36, 1358 (2007)

[Related Articles]

[1] Hollman PC, Katan MB, Biomed. Pharmacother., 51, 305 (1997)

[2] Jeong EJ, Cha YJ, J. Life Sci., 22, 1665 (2012)

[3] Hyun MR, Lee YS, Park YH, Kor. J. Hort. Sci. Technol., 29, 68 (2011)

[4] Cheigh CI, Yoo SY, Chung MS, Kor. J. Food & Nutr., 24, 458 (2011)

[5] Lee HJ, Park SN, J. Soc.Cosmet. Sci. Korea, 37, 143 (2011)

[6] Li R, Yuan C, Dong C, Shuang S, Choi MMF, N. Schmied. Arch. Pharmacol., 383, 437 (2011)

[7] Rogerio AP, Kanashiro A, Fontanari C, da Silva EV, Lucisano-Valim YM, Soares EG, Faccioli LH, Inflamm. Res., 56, 402 (2007)

[8] Gasparotto Junior A , Gasparotto FM, Lourenco ELB, Crestani S, Stefanello MEA, Salvador MJ, da Silva-Santos JE, Marques MCA, Kassuya CAL, J. Ethnopharmacol., 134, 363 (2011)

[9] Inaba H, Tagashira M, Honma D, Kanda T, Kou Y, Ohtake Y, Amano A, Biol. Pharm. Bull., 31, 527 (2008)

[10] Amado NG, Cerqueira DM, Menezes FS, Mendes da Silva JF, Neto Vivaldo M, Abreu JG, Anti Canc. Drugs, 20, 543 (2009)

[11] Williams BL, Wender SH, Arch.Biochem. Biophys., 43, 319 (1953)

[12] Wang J, Lu D, Zhao H, Jiang B, Wang J, Ling X, Chai H, Ouyang P, J. Serb. Chem. Soc., 75, 875 (2010)

[13] Mauludin R, Muller RH, Keck CM, Int. J. Pharm., 370, 202 (2009)

[14] Wang J, Zhao LL, Sun GX, Liang Y, Wu FA, Chen Zl, Cui SM, Afr. J. Biotechnol., 10, 1460 (2011)

[15] Chebil L, Humeau C, Anthoni J, Dehez F, Engasser JM, Ghoul M, J. Chem. Eng. Data, 52(5), 1552 (2007)

[16] You HJ, Ahn HJ, Ji GE, Agric.Food Chem., 58, 10886 (2010)

[17] Weignerova L, Marhol P, Gerstorferova D, Kren V, Bioresource Tech., 115, 222 (2012)

[18] HB Lee, EK Kim, SJ Park, SG Bang, TG Kim, DW Chung, J. Agric. Food Chem., 58, 4808 (2010)

[19] DW Chung, SB Lee, J. Sci. Food Agric., 93, 362 (2013)

[20] HB Lee, EK Kim, SJ Park, SG Bang, TG Kim, DW Chung, J. Sci. Food Agric., 91, 2315 (2011)

[21] Lee SB, Chung DW, Appl. Chem. Eng., 23(4), 394 (2012)

[22] Lee MH, Huh D, Jo DJ, Lee GD, Yoon SR, J. Korean Soc. Food Sci. Nutr., 36, 1358 (2007)