Fabrication of Three-Dimensional Poly(lactic-co-glycolic acid) Mesh by Electrospinning Using Different Solvents with Dry Ice

Hye-Lee Kim; Jeong-Hyun Lee; Hyok Jin Seo; Kyung Eun You; Mi Hee Lee; Jong-Chul Park

Macromolecular Research, Vol.22, No.4, 377-381, April, 2014

DOI10.1007/s13233-014-2060-7 Export Citation

Fabrication of Three-Dimensional Poly(lactic-co-glycolic acid) Mesh by Electrospinning Using Different Solvents with Dry Ice

Hye-Lee Kim^{1, 2}, Jeong-Hyun Lee^{1, 2}, Hyok Jin Seo^{1, 2}, Kyung Eun You^{1, 2}, Mi Hee Lee^{1, 3}, and Jong-Chul Park^{1, 2, †}

¹Department of Medical Engineering, Yonsei University College of Medicine, Seoul, 120-752, Korea
²Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, 120-752, Korea
³, Korea

E-mail:

Scaffolds used in tissue engineering are usually porous for providing a passage and cell infiltration. In this study, we have investigated the effects of vapor pressure of solvents during electrospinning with dry ice on the pore sizes of the poly(lactic-co-glycolic acid) (PLGA) meshes and the cell behaviors in these meshes. The meshes fabricated with dry ice using 1,1,1,3,3,3,-hexafluoro-2-propanol (HFIP), which was the lowest vapor pressure of the experimental solvents, were not significantly different on the fiber connections as compared to those fabricated without dry ice. When electrospinning was processed using acetone with dry ice, numerous beads were observed in the meshes. The meshes which were fabricated using the tetrahydrofuran/dimethylformamide (THF/DMF) admixture with dry ice had loose connections between the fibers and showed large voids. In addition, the infiltration and the attachment of cells increased on these meshes due to large voids. Therefore, dry ice during electrospinning could cause an increase in pore size; however, these electrospinning processing was sensitive to the vapor pressure of the experimental solven.

Keywords:electrospinning;dry ice;vapor pressure of solvents;large por

[References]

Yoon JJ, Park TG, J. Biomed. Mater. Res. B: Appl. Biomater., 55, 401 (2001)
Miller DC, Thapa A, Haberstroh KM, Webster TJ, Biomaterials, 25, 53 (2004)
Kuila BK, Nandi AK, Macromolecules, 37(23), 8577 (2004)
Luong ND, Moon IS, Nam JD, Macromol. Mater. Eng., 294, 699 (2009)
Nie L, Chen D, Suo J, Zou P, Feng S, Yang Q, Yang S, Ye S, Colloids Surf. B: Biointerfaces, 100, 169 (2012)
Stitzel J, Liu J, Lee SJ, Komura M, Berry J, Soker S, Lim G, Dyke MV, Czerw R, Yoo JJ, Atala A, Biomaterials, 27, 1088 (2006)
Pham QP, Sharma U, Mikos AG, Tissue Eng., 12, 1197 (2006)
Chen JP, Chang GY, Chen JK, Colloids Surf. A:Physicochem. Eng. Asp., 313-314, 183 (2008)
Schneider OD, Loher S, Brunner TJ, Uebersax J, Simonet M, Grass RN, Markle HP, Stark WJ, J. Biomed.Mater. Res. Part B: Appl. Biomater., 84, 350 (2008)
Fu L, Liu Y, Hu P, Xiao K, Yu G, Zhu D, Chem. Mater., 15, 4287 (2003)
Mutsuga M, Narita Y, Yamawaki A, Satake M, Kaneko H, Suematsu Y, Usui A, Ueda Y, J. Thorac. Cardiovasc. Surg., 137, 703 (2009)
Simonet M, Schneider OD, Neuenschwander P, Stark WJ, Polym. Eng. Sci., 47(12), 2020 (2007)
Leong MF, Rasheed MZ, Lim TC, Chian KS, J. Biomed. Mater. Res., 91, 231 (2009)
Kim HL, Lee JH, Lee MH, Kwon BJ, Park JC, Tissue Eng. Part A., 18, 2315 (2012)
Alhosseini SN, Moztarzadeh F, Mozafari M, Asgari S, Dodel M, Samadikuchaksaraei A, Kargoza S, Jalali N, Int. J. Nanomedicine., 7, 25 (2012)
Xu CC, Chan RW, Tirunaga N, Tissue Eng., 13, 551 (2007)
Greiner A, Wendorff JH, Angew. Chem. Int. Ed., 46, 2 (2007)
Casper CL, Stephens JS, Tassi NG, Chase DB, Rabolt JF, Macromolecules, 37(2), 573 (2004)
Doshi J, Reneker DH, J. Electrostat., 35, 151 (1995)

[1] Yoon JJ, Park TG, J. Biomed. Mater. Res. B: Appl. Biomater., 55, 401 (2001)

[2] Miller DC, Thapa A, Haberstroh KM, Webster TJ, Biomaterials, 25, 53 (2004)

[3] Kuila BK, Nandi AK, Macromolecules, 37(23), 8577 (2004)

[4] Luong ND, Moon IS, Nam JD, Macromol. Mater. Eng., 294, 699 (2009)

[5] Nie L, Chen D, Suo J, Zou P, Feng S, Yang Q, Yang S, Ye S, Colloids Surf. B: Biointerfaces, 100, 169 (2012)

[6] Stitzel J, Liu J, Lee SJ, Komura M, Berry J, Soker S, Lim G, Dyke MV, Czerw R, Yoo JJ, Atala A, Biomaterials, 27, 1088 (2006)

[7] Pham QP, Sharma U, Mikos AG, Tissue Eng., 12, 1197 (2006)

[8] Chen JP, Chang GY, Chen JK, Colloids Surf. A:Physicochem. Eng. Asp., 313-314, 183 (2008)

[9] Schneider OD, Loher S, Brunner TJ, Uebersax J, Simonet M, Grass RN, Markle HP, Stark WJ, J. Biomed.Mater. Res. Part B: Appl. Biomater., 84, 350 (2008)

[10] Fu L, Liu Y, Hu P, Xiao K, Yu G, Zhu D, Chem. Mater., 15, 4287 (2003)

[11] Mutsuga M, Narita Y, Yamawaki A, Satake M, Kaneko H, Suematsu Y, Usui A, Ueda Y, J. Thorac. Cardiovasc. Surg., 137, 703 (2009)

[12] Simonet M, Schneider OD, Neuenschwander P, Stark WJ, Polym. Eng. Sci., 47(12), 2020 (2007)

[13] Leong MF, Rasheed MZ, Lim TC, Chian KS, J. Biomed. Mater. Res., 91, 231 (2009)

[14] Kim HL, Lee JH, Lee MH, Kwon BJ, Park JC, Tissue Eng. Part A., 18, 2315 (2012)

[15] Alhosseini SN, Moztarzadeh F, Mozafari M, Asgari S, Dodel M, Samadikuchaksaraei A, Kargoza S, Jalali N, Int. J. Nanomedicine., 7, 25 (2012)

[16] Xu CC, Chan RW, Tirunaga N, Tissue Eng., 13, 551 (2007)

[17] Greiner A, Wendorff JH, Angew. Chem. Int. Ed., 46, 2 (2007)

[18] Casper CL, Stephens JS, Tassi NG, Chase DB, Rabolt JF, Macromolecules, 37(2), 573 (2004)

[19] Doshi J, Reneker DH, J. Electrostat., 35, 151 (1995)