화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.22, No.5, 461-473, May, 2014
DOI10.1007/s13233-014-2080-3  Export Citation
Combination of Conjugated Polyelectrolytes and Biomolecules: A New Optical Platform for Highly Sensitive and Selective Chemo- and Biosensors
Ji-Eun Jeong, Shin-Jae Woo, Van Sang Le, Hyejin Choi, and Han Young Woo†
  • Department of Cogno-Mechatronics Engineering, Pusan National University, Gyeongnam, 627-706, Korea
E-mail:
Conjugated polyelectrolytes (CPEs) have been studied extensively as the optical platform for chemo- and biosensor applications with high sensitivity by virtue of their light harvesting effect. A range of fluorescent CPEsbased sensing schemes have been developed by utilizing fluorescence quenching via photo-induced electron transfer and fluorescence resonance energy transfer, etc. Highly sensitive and selective detection assays can be realized by combining the tunable optical properties of synthetic CPEs with specific binding characteristics of biomolecules (such as aptamer, antibody, protein, etc.). This article reviews the range of fluorescence-based optical assays with the CPEs-biomolecule complex as a signaling platform with in situ detection, remarkable sensitivity and selectivity, etc.
Keywords:conjugated polyelectrolytes;fluorescence;biosensor;CPE;fluorescence resonance energy transfer;selectivity.
  1. McQuade DT, Pullen AE, Swager TM, Chem. Rev., 100(7), 2537 (2000)
  2. Thomas SW, Joly GD, Swager TM, Chem. Rev., 107(4), 1339 (2007)
  3. Lee BH, Jung IH, Woo HY, Shim HK, Kim G, Lee K, Adv. Funct. Mater., 24, 1100 (2013)
  4. Lee W, Seo JH, Woo HY, Polymer, 54(19), 5104 (2013)
  5. Zhou Q, Swager TM, J. Am. Chem. Soc., 117(26), 7017 (1995)
  6. Shin YC, Lee JH, Jeong JE, Kim B, Lee EJ, Jin OS, Jung TG, Lee JJ, Woo HY, Han DW, Biotechnol. Appl. Biochem., 60, 580 (2013)
  7. Duarte A, Pu KY, Liu B, Bazan GC, Chem. Mater., 23, 501 (2011)
  8. Chen L, McBranch DW, Wang HL, Helgeson R, Wudi F, Whitten DG, Proc. Natl. Acad. Sci. U.S.A., 96, 12287 (1999)
  9. Lee K, Povlich LK, Kim J, Adv. Funct. Mater., 17(14), 2580 (2007)
  10. Le VS, Kim B, Lee W, Jeong JE, Yang RQ, Woo HY, Macromol. Rapid Commun., 34(9), 772 (2013)
  11. Nguyen BL, Jeong JE, Jung IH, Kim B, Le VS, Kim I, Kyhm K, Woo HY, Adv. Funct. Mater. (2013). DOI: 10.1002/adfm.201301515 (in press)
  12. You J, Kim J, Park T, Kim B, Kim E, Adv. Funct. Mater., 22(7), 1417 (2012)
  13. Gaylord BS, Heeger AJ, Bazan GC, Proc. Natl. Acad. Sci. U.S.A., 99, 10954 (2002)
  14. Gaylord BS, massie MR, Feinstein SC, Bazan GC, Proc. Natl. Acad. Sci. U.S.A., 102, 34 (2005)
  15. Ellington AD, Szostak JW, Nature, 346, 818 (1990)
  16. Hermann T, Patel DJ, Science, 287, 820 (2000)
  17. Hamaguchi N, Ellington A, Stanton M, Anal. Biochem., 294, 126 (2001)
  18. Jayasena SD, Clin. Chem., 45, 1628 (1999)
  19. Stoltenburg R, Reinemann C, Strehlitz B, Biomol. Eng., 24, 381 (2007)
  20. Burbulis I, Yamaguchi K, Yu R, Resnekov O, Brent R, Nature Methods, 4, 1011 (2007)
  21. Kuo HC, Cheng CF, Clark RB, Lin JJC, Lin JLC, Hoshijima M, Nguyen-Tran VTB, Gu Y, Ikeda Y, Chu PH, Ross J, Giles WR, Chien KR, Cell, 107, 801 (2001)
  22. Walz W, Neurochem. Int., 36, 291 (2000)
  23. Teresa M, Gomes SR, Tavares KS, Oliveira JABP, Analyst, 125, 1983 (2000)
  24. He HR, Mortellaro MA, Leiner MJP, Fraatz RJ, Tusa JK, J. Am. Chem. Soc., 125(6), 1468 (2003)
  25. Williamson JR, Curr. Opin. Struct. Biol., 3, 357 (1993)
  26. Neidle S, Parkinson G, Nat. Rev. Drug Discov., 1, 383 (2002)
  27. Arthanari H, Bolton PH, Chem. Biol., 8, 221 (2001)
  28. Gilbert DE, Feigon J, Curr. Opin. Struct. Biol., 9, 305 (1999)
  29. Siddiqui-Jain A, Grand CL, Bearss DJ, Hurley LH, Proc. Natl. Acad. Sci. U.S.A., 99, 11583 (2002)
  30. He F, Tang YL, Yu MH, Feng F, An LL, Sun H, Wang S, Li YL, Zhu DB, Bazan GC, J. Am. Chem. Soc., 128(21), 6764 (2006)
  31. Xu H, Gao SL, Yang Q, Pan D, Wang LH, Fan CH, Acs Appl. Mater. Interfaces, 2, 3211 (2010)
  32. Ho HA, Leclerc M, J. Am. Chem. Soc., 126(5), 1384 (2004)
  33. Kim B, Jung IH, Kang M, Shim HK, Woo HY, J. Am. Chem. Soc., 134(6), 3133 (2012)
  34. Bowman LJ, Anderson CD, Chapman WC, Semin. Thromb. Hemost., 36, 477 (2010)
  35. Nishino A, Suzuki M, Ohtani H, Motohashi O, Umezawa K, Nagura H, Yoshimoto T, J. Neurotrauma, 10, 167 (1993)
  36. Inuyama H, Saito T, Takagi J, Saito Y, J. Cell. Physio., 173, 406 (1997)
  37. Pu F, Huang Z, Hu D, Ren J, Wang S, Qu X, Chem. Commun., 7357 (2009)
  38. Yan SY, Huang R, Zhou Y, Zhang M, Deng M, Wang X, Weng X, Zhou X, Chem. Commun., 47, 1273 (2011)
  39. Wang YY, Liu B, Langmuir, 25(21), 12787 (2009)
  40. Wang YY, Pu KY, Liu B, Langmuir, 26(12), 10025 (2010)
  41. Xia F, Zuo XL, Yang RQ, Xiao Y, Kang D, Vallee-Belisle A, Gong X, Heeger AJ, Plaxco KW, J. Am. Chem. Soc., 132(4), 1252 (2010)
  42. Stojanovic MN, de Prada P, Landry DW, J. Am. Chem. Soc., 123(21), 4928 (2001)
  43. Xia F, Zuo X, Yang R, Xiao Y, Kang D, Vallee-Belisle A, Gong X, Yuen JD, Hsu BBY, Heeger AJ, Plaxco KW, Proc. Natl. Acad. Sci. U.S.A., 107, 10837 (2010)
  44. Li B, Qin C, Li T, Wang L, Dong S, Anal. Chem., 81, 3544 (2009)
  45. Litman GW, Rast JP, Shamblott MJ, Haire RN, Hulst M, Roess W, Litman RT, Hinds-Frey KR, Zilch A, Amemiya CT, Mol. Biol. Evol., 10, 60 (1993)
  46. Petrou PS, Mastichiadis C, Christofidis I, Kakabakos SE, Anal. Chem., 79, 647 (2007)
  47. Wang J, Liu G, Engelhard MH, Lin Y, Anal. Chem., 78, 6974 (2006)
  48. Zhang X, Wu Y, Tu Y, Liu S, Analyst, 133, 485 (2008)
  49. Wan LS, Ke BB, Xu ZK, Enzyme Microb. Technol., 42(4), 332 (2008)
  50. Lee K, Lee J, Jeong EJ, Kronk A, Elenitoba-Johnson KSJ, Lim MS, Kim J, Adv. Mater., 24(18), 2479 (2012)
  51. Wu CF, Schneider T, Zeigler M, Yu JB, Schiro PG, Burnham DR, McNeill JD, Chiu DT, J. Am. Chem. Soc., 132(43), 15410 (2010)
  52. Wang YY, Liu B, Biosens. Bioelectron., 24, 3293 (2009)
  53. Wang D, Gong X, Heeger PS, Rininsland F, Bazan GC, Heeger AJ, Proc. Natl. Acad. Sci. U.S.A., 99, 49 (2002)
  54. Gutteridge A, Thornton JM, Trends Biochem. Sci., 30, 622 (2005)
  55. Fulton AB, Isaacs WB, Bioessays, 13, 157 (1991)
  56. Westermeier R, Marouga R, Biosci. Rep., 25, 19 (2005)
  57. Green NM, Advan. Protein Chem., 29, 85 (1975)
  58. Holmberg A, Blomstergren A, Nord O, Lukacs M, Lundeberg J, Uhlen M, Electrophoresis, 26(3), 501 (2005)
  59. Ji EK, Wu DL, Schanze KS, Langmuir, 26(18), 14427 (2010)
  60. An LL, Tang YL, Wang S, Li YL, Zhu DB, Macromol. Rapid Commun., 27(13), 993 (2006)
  61. Sharon N, Lis H, Science, 177, 949 (1972)
  62. Lee YC, Lee RT, Acc. Chem. Res., 28, 321 (1995)
  63. Dwek RA, Chem. Rev., 96(2), 683 (1996)
  64. Lis H, Sharon N, Chem. Rev., 98(2), 637 (1998)
  65. Simanek EE, McGarvey GJ, Jablonowski JA, Wong CH, Chem. Rev., 98(2), 833 (1998)
  66. Shi J, Cai L, Pu KY, Liu B, Chem. Asian J., 5, 301 (2010)
  67. Pu KY, Shi JB, Wang LH, Cai LP, Wang GA, Liu B, Macromolecules, 43(23), 9690 (2010)