On-plate spot test for identification and separation of benzalkonium chloride and cetrimide in drug samples: A mechanistic view

Ali Mohammad; Asma Siddiq; Rizwana Mobin; Gaber E.- El-Desoky

Journal of Industrial and Engineering Chemistry, Vol.20, No.4, 1697-1702, July, 2014

DOI10.1016/j.jiec.2013.08.019 Export Citation

On-plate spot test for identification and separation of benzalkonium chloride and cetrimide in drug samples: A mechanistic view

Ali Mohammad^†, Asma Siddiq, Rizwana Mobin, and Gaber E.- El-Desoky¹

Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh, India
¹Department of Chemistry, College of Science, Building 5, King Saud University, Riyadh, Saudi Arabia

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A mechanism showing different modes of interaction of benzalkonium chloride and cetrimide with the stationary and mobile phase has been proposed which leads to the separation of this surfactant mixture. Other combination of cationic surfactants was also separated from their binary mixture by the chromatographic system comprising of silica gel and combination of green solvents as mobile phase. The effect of presence of impurities on separation process was also examined and the limits of detection of the separated surfactants were calculated. The developed method was also successfully applied for the identification of cetrimide and benzalkonium chloride in pharmaceutical formulations.

Keywords:Separation;Benzalkonium chloride;Cetrimide;Densitometry;Pharmaceutical formulations

[References]

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Migahed MA, Abd-El-Raouf M, Al-Sabagh AM, Abd-El-Bary HM, Electrochim. Acta, 50(24), 4683 (2005)
Hegazy MA, Abdallah M, Ahmed H, Corrosion Sci., 52, 2897 (2010)
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Castillo JA, Clape’s P, Infante MR, Comas J, Manresa A, J. Antimicrob. Chemoter., 57(5), 691 (2006)
Milligan BG, Plant Mol. Biol. Rep., 7, 144 (1989)
Mohammad A, Haq N, Siddiq A, J. Sep. Sci., 33, 3619 (2010)
Mohammad A, Siddiq A, Moheman A, El-Desoky GE, J. Planar Chromatogr., 26, 31 (2013)
Mohammad A, Bhawani SA, J. Chromatogr. Sci., 46, 298 (2008)
Mohammad A, Siddiq A, El-Desoky GE, J. Anal. Sci. Technol., 4, 2 (2013)
Siddiq A, Ansari MO, Mohammad A, Mohammad F, El-Desoky GE, Int. J. Polym. Mater. Polym. Biomater. (2013)
Bhawani SA, Sulaiman O, Hashim R, Mohamad Ibrahim MN, Tenside Surfactants Deterg., 2, 73 (2010)
Stahl E, Thin Layer Chromatography, A Laboratory Handbook, Springer, Springer-Verlag, Heidelberg, Berlin, 1969.
Synder LR, in: Heftmann E (Ed.), Adsorption in Chromatography, third ed., Reinhold, New York, 1975.

[1] Puchta R, J. Am. Oil Chem. Soc., 61, 367 (1984)

[2] Migahed MA, Abd-El-Raouf M, Al-Sabagh AM, Abd-El-Bary HM, Electrochim. Acta, 50(24), 4683 (2005)

[3] Hegazy MA, Abdallah M, Ahmed H, Corrosion Sci., 52, 2897 (2010)

[4] Leung S, Gironella A, Trigo C, Bhushan A, Daniels CK, Lai JC, Proc. Inst. Mech. Eng., 22, 153 (2007)

[5] Castillo JA, Clape’s P, Infante MR, Comas J, Manresa A, J. Antimicrob. Chemoter., 57(5), 691 (2006)

[6] Milligan BG, Plant Mol. Biol. Rep., 7, 144 (1989)

[7] Mohammad A, Haq N, Siddiq A, J. Sep. Sci., 33, 3619 (2010)

[8] Mohammad A, Siddiq A, Moheman A, El-Desoky GE, J. Planar Chromatogr., 26, 31 (2013)

[9] Mohammad A, Bhawani SA, J. Chromatogr. Sci., 46, 298 (2008)

[10] Mohammad A, Siddiq A, El-Desoky GE, J. Anal. Sci. Technol., 4, 2 (2013)

[11] Siddiq A, Ansari MO, Mohammad A, Mohammad F, El-Desoky GE, Int. J. Polym. Mater. Polym. Biomater. (2013)

[12] Bhawani SA, Sulaiman O, Hashim R, Mohamad Ibrahim MN, Tenside Surfactants Deterg., 2, 73 (2010)

[13] Stahl E, Thin Layer Chromatography, A Laboratory Handbook, Springer, Springer-Verlag, Heidelberg, Berlin, 1969.

[14] Synder LR, in: Heftmann E (Ed.), Adsorption in Chromatography, third ed., Reinhold, New York, 1975.