Silane graft copolymer modified with sulphonic acid functional groups used for immobilization of trypsin from aqueous solutions via adsorption process

T.S. Anirudhan; Tharun A. Rauf

Journal of Industrial and Engineering Chemistry, Vol.20, No.4, 1901-1910, July, 2014

DOI10.1016/j.jiec.2013.09.009 Export Citation

Silane graft copolymer modified with sulphonic acid functional groups used for immobilization of trypsin from aqueous solutions via adsorption process

T.S. Anirudhan^†, and Tharun A. Rauf

Department of Chemistry, University of Kerala, Kariavattom, Trivandrum 695 581, India

E-mail:

A novel silane based adsorbent material, [stearyl alcohol (SA)-grafted-epichlorohydrin (E)]-grafted-aminoproypyl silanetriol (APST) was synthesized and functionalized with sulfonic acid [(SA-g-E)-g-APST/SO3H]. The adsorbent material was characterized by FTIR, XRD, SEM and BET analysis. Protein. particle interaction was studied by the immobilization of trypsin (TRY) using batch adsorption process. Maximum adsorption was attained at pH 7.0. Adsorption process followed pseudo-second-order kinetics; film diffusion controlled and Sips isotherm mechanism. Evaluation of separation factor (RL) and thermodynamic parameters revealed the favorability of adsorption process. Adsorption/desorption studies, for over six cycles showed the repeatability and regeneration capability of the adsorbent.

Keywords:Double grafting;Silane;Sulfonic acid;Trypsin;Adsorption/desorption

[References]

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Bayramoglu G, Yilmaz M, Arica MY, Food Chem., 84, 591 (2004)
Sun H, Hong J, Meng F, Gong P, Yu J, Xue Y, Zhao S, Xu D, Dong L, Yao S, Coat. Technol., 201, 250 (2006)
Arica MY, Bayramoglu G, J. Mol. Catal. B-Enzym., 27, 255 (2004)
Yildiz HB, Sahmetlioglu E, Boyukbayram AE, Toppare L, Yagci Y, Int. J. Biol. Macromol., 41, 332 (2007)
Matsuno H, Nagasaka Y, Kurita K, Serizawa T, Chem. Mater., 19, 2174 (2007)
Lee JA, Lee WK, J. Ind. Eng. Chem., 19(5), 1448 (2013)
Liu CG, Chen XG, Park HJ, Carbohydr. Polym., 62, 293 (2005)
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Goddard JM, Hotchkiss JH, Prog. Polym. Sci, 32, 698 (2007)
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Standeker S, Veronovski A, Novak Z, Knez Z, Desalination, 269(1-3), 223 (2011)
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Koskella J, Stotzky G, Appl. Environ. Microbiol., 63, 3561 (1997)
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Kang HC, Lee BM, Yoon M, J. Am. Oil Chem. Soc., 78, 423 (2001)
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Schwarz JA, Driscoll CT, Bhanot AK, J. Colloid Interface Sci., 97, 55 (1984)
Shubha KP, Raji C, Anirudhan TS, Water Res., 35, 300 (2001)
Kyung JL, Jung TP, Joo HK, Byoung RM, Jong HK, Ionics, 15, 163 (2009)
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Makoto O, Yuya F, Yukihiro O, Colloids Surf. B: Biointerfaces, 69, 194 (2009)
Prestrelski SJ, Byler DM, Liebman MN, Biochemistry, 30, 133 (1991)
Yokoi T, Yoshitake H, Yamada T, Kubota Y, Tatsumi T, J. Mater. Chem., 16, 1125 (2006)
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Anirudhan TS, Suchithra PS, Ind. Eng. Chem. Res., 46(13), 4606 (2007)
Anabela L, Rosalina S, Adsorption, 11, 167 (2005)
Khan MA, Ngabura M, Choong TSY, Masood H, Chuah LA, Bioresour. Technol., 103(1), 35 (2012)
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[Referenced By]

[1] Maliar T, Jedinak A, Kadrabova J, Sturdik E, Eur. J. Med. Chem., 39, 241 (2004)

[2] Wang DM, Sun Y, Biochem. Eng. J., 37, 332 (2007)

[3] Miyoshi K, Saito K, Shiraishi T, Sugo T, J. Membr. Sci., 264(1-2), 97 (2005)

[4] Jansson M, Johansson HO, Colloids Surf. B: Biointerfaces, 37, 71 (2004)

[5] Koutsopoulos S, Patzsch K, Bosker WTE, Norde W, Langmuir, 23(4), 2000 (2007)

[6] Norde W, Lyklema J, J. Colloid Interface Sci., 71, 350 (1979)

[7] Bayramoglu G, Yilmaz M, Arica MY, Food Chem., 84, 591 (2004)

[8] Sun H, Hong J, Meng F, Gong P, Yu J, Xue Y, Zhao S, Xu D, Dong L, Yao S, Coat. Technol., 201, 250 (2006)

[9] Arica MY, Bayramoglu G, J. Mol. Catal. B-Enzym., 27, 255 (2004)

[10] Yildiz HB, Sahmetlioglu E, Boyukbayram AE, Toppare L, Yagci Y, Int. J. Biol. Macromol., 41, 332 (2007)

[11] Matsuno H, Nagasaka Y, Kurita K, Serizawa T, Chem. Mater., 19, 2174 (2007)

[12] Lee JA, Lee WK, J. Ind. Eng. Chem., 19(5), 1448 (2013)

[13] Liu CG, Chen XG, Park HJ, Carbohydr. Polym., 62, 293 (2005)

[14] Wang F, Guoa C, Liu HZ, Liu CZ, J. Mol. Catal. B-Enzym., 48, 1 (2007)

[15] Goddard JM, Hotchkiss JH, Prog. Polym. Sci, 32, 698 (2007)

[16] Aggarwal P, Hall JB, McLeland CB, Dobrovolskaia MA, McNeil SE, Adv. Drug Deliv. Rev., 61, 428 (2009)

[17] Anirudhan TS, Rauf TA, J. Ind. Eng. Chem., 19(5), 1659 (2013)

[18] Standeker S, Veronovski A, Novak Z, Knez Z, Desalination, 269(1-3), 223 (2011)

[19] Demaneche S, Jocteur-Monrozier L, Quiquampoix H, Simonet P, Appl. Environ. Microbiol., 67, 293 (2001)

[20] Koskella J, Stotzky G, Appl. Environ. Microbiol., 63, 3561 (1997)

[21] Mahajan R, Burns R, Schaeffer M, Koros WJ, J. Appl. Polym. Sci., 86(4), 881 (2002)

[22] Kang HC, Lee BM, Yoon M, J. Am. Oil Chem. Soc., 78, 423 (2001)

[23] Fabian M, Daberkow T, Treccani L, Wilhelm M, Schowalter M, Rosenauer A, Madler L, Rezwan K, Acta Biomater., 8, 1221 (2012)

[24] Schwarz JA, Driscoll CT, Bhanot AK, J. Colloid Interface Sci., 97, 55 (1984)

[25] Shubha KP, Raji C, Anirudhan TS, Water Res., 35, 300 (2001)

[26] Kyung JL, Jung TP, Joo HK, Byoung RM, Jong HK, Ionics, 15, 163 (2009)

[27] Wieslaw WS, Krzysztof N, Anna S, Agnieszka W, Wioletta MB, Danuta P, Barbara M, Pure Appl. Chem., 81, 2417 (2009)

[28] Makoto O, Yuya F, Yukihiro O, Colloids Surf. B: Biointerfaces, 69, 194 (2009)

[29] Prestrelski SJ, Byler DM, Liebman MN, Biochemistry, 30, 133 (1991)

[30] Yokoi T, Yoshitake H, Yamada T, Kubota Y, Tatsumi T, J. Mater. Chem., 16, 1125 (2006)

[31] Russo PA, Ribeiro Carrott MML, Mourao PAM, Carrott PJM, Colloids Surf. A: Physicochem. Eng. Asp., 386, 25 (2011)

[32] Arun KB, Venkobachar C, J. Environ. Eng., 110, 110 (1984)

[33] Anirudhan TS, Suchithra PS, Ind. Eng. Chem. Res., 46(13), 4606 (2007)

[34] Anabela L, Rosalina S, Adsorption, 11, 167 (2005)

[35] Khan MA, Ngabura M, Choong TSY, Masood H, Chuah LA, Bioresour. Technol., 103(1), 35 (2012)

[36] Vargas AMM, Cazetta AL, Kunita MH, Silva TL, Almeida VC, Chem. Eng. J., 168(2), 722 (2011)

[37] Ruthven DM, Principles of Adsorption and Adsorption Processes, Wiley, New York, 1984.

[38] Almeida CAP, Debacher NA, Downs AJ, Cottet L, Mello CAD, J. Colloid Interface Sci., 332(1), 46 (2009)