Cellular Response of Silk Fibroin Nanofibers Containing Silver Nanoparticles In vitro

Lim Jeong; Donghwan Cho; Oh Hyeong Kwon; Byung-Moo Min; Won Ho Park

Macromolecular Research, Vol.22, No.7, 796-803, July, 2014

DOI10.1007/s13233-014-2104-z Export Citation

Cellular Response of Silk Fibroin Nanofibers Containing Silver Nanoparticles In vitro

Lim Jeong, Donghwan Cho¹, Oh Hyeong Kwon¹, Byung-Moo Min^{2, †}, and Won Ho Park^†

Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon, 305-764, Korea
¹Department of Polymer Science and Engineering, Kumoh Institute of Technology, Gyeongbuk, 730-701, Korea
²Department of Oral Biochemistry, Dental Research Institute, Seoul National University, School of Dentistry, Seoul, 110-744, Korea

E-mail:,

Silk Fibroin (SF) nanofibers containing various silver compounds, such as silver tetrafluoroborate, silver phosphate, silver sulfadiazine, and silver acetate, were prepared by electrospinning. Silver compounds were dissolved into SF solution in 98% formic acid. In this system, the formic acid was used both as a co-solvent of SF and silver compounds, and as a reducing agent for silver ions in solution. The average diameters of SF nanofibers varied depending on the type of silver compounds added. The Ag element in the SF nanofibers was confirmed to be mainly Ag nanoparticles by XPS results. The in vitro cytotoxicity of silver compounds in SF nanofibers on normal human keratinocytes and fibroblasts was examined. Overall, the silver compounds in SF nanofibers are cytotoxic to both normal human epidermal keratinocytes (NHEK) and normal human epidermal fibroblasts (NHEF) cells.

Keywords:silk fibroin nanofibers;Ag nanoparticles;epidermal cells;cytotoxicity.

[References]

Vepari C, Kaplan DL, Prog. Polym. Sci., 32, 991 (2007)
Kundu B, Rajkhowa R, Kundu SC, Wang X, Adv. Drug Deliv. Rev., 65, 457 (2013)
Jeong L, Lee KY, Park WH, Key Eng. Mater., 342, 813 (2007)
Zarkoob S, Eby RK, Reneker DH, Hudson SD, Ertley D, Adams WW, Polymer, 45(11), 3973 (2004)
Min BM, Jeong L, Lee KY, Park WH, Macromol. Biosci., 6, 285 (2006)
Jeong L, Lee KY, Liu JW, Park WH, Int. J. Biol. Macromol., 38, 40 (2006)
Ohgo K, Zhao CH, Kobayashi M, Asakura T, Polymer, 44(3), 841 (2003)
Kim SH, Nam YS, Lee TS, Park WH, Polym. J., 35, 185 (2003)
Ayutsede J, Gandhi M, Sukigara S, Micklus M, Chen HE, Ko F, Polymer, 46(5), 1625 (2005)
Wang H, Shao HL, Hu XC, J. Appl. Polym. Sci., 101(2), 961 (2006)
Jin HJ, Chen J, Karageorgiou V, Altman GH, Kaplan DL, Biomaterials, 25, 1039 (2004)
Lee KH, Ki CS, Baek DH, Kang GD, Ihm DW, Park YH, Fibers Polym., 6, 181 (2005)
Li C, Vepari C, Jin HJ, Kim HJ, Kaplan DL, Biomaterials, 27, 3115 (2006)
Kang M, Jung R, Kim HS, Youk JH, Jin HJ, J. Nanosci. Nanotechnol., 7, 3888 (2007)
Li WL, Wang JJ, Chi HX, Wei GZ, Zhang J, Dai LX, J. Appl. Polym. Sci., 123(1), 20 (2012)
Kang M, Jin HJ, Colloid Polym. Sci., 285, 1163 (2007)
Zhang YZ, Venugopal J, Huang ZM, Lim CT, Ramakrishna S, Polymer, 47(8), 2911 (2006)
Chun JY, Kang HK, Jeong L, Kang YO, Oh JE, Yeo IS, Jung SY, Park WH, Min BM, Colloid Surf. B: Biointerfaces, 78, 334 (2010)
Min BM, Lee G, Kim SH, Nam YS, Lee TS, Park WH, Biomaterials, 25, 1289 (2004)
Mould AP, Askari JA, Humphries MJ, J. Biol. Chem., 275, 20324 (2000)
Romanyuk A, Oelhafen P, Sol. Energy Mater. Sol. Cells, 91(12), 1051 (2007)
Pal T, Curr. Sci., 83, 627 (2002)
Atiyeh BS, Costagliola M, Hayek SN, Dibo SA, Burns, 33, 139 (2007)
Poon VK, Burd A, Burns, 30, 140 (2004)
Hidalgo E, Bartolome R, Barroso C, Moreno A, Dominguez C, Skin Pharmacol. Physiol., 11, 140 (1998)
Hidalgo E, Domlnguez C, Toxicol. Lett., 98, 169 (1998)

[Referenced By]

[Related Articles]

[1] Vepari C, Kaplan DL, Prog. Polym. Sci., 32, 991 (2007)

[2] Kundu B, Rajkhowa R, Kundu SC, Wang X, Adv. Drug Deliv. Rev., 65, 457 (2013)

[3] Jeong L, Lee KY, Park WH, Key Eng. Mater., 342, 813 (2007)

[4] Zarkoob S, Eby RK, Reneker DH, Hudson SD, Ertley D, Adams WW, Polymer, 45(11), 3973 (2004)

[5] Min BM, Jeong L, Lee KY, Park WH, Macromol. Biosci., 6, 285 (2006)

[6] Jeong L, Lee KY, Liu JW, Park WH, Int. J. Biol. Macromol., 38, 40 (2006)

[7] Ohgo K, Zhao CH, Kobayashi M, Asakura T, Polymer, 44(3), 841 (2003)

[8] Kim SH, Nam YS, Lee TS, Park WH, Polym. J., 35, 185 (2003)

[9] Ayutsede J, Gandhi M, Sukigara S, Micklus M, Chen HE, Ko F, Polymer, 46(5), 1625 (2005)

[10] Wang H, Shao HL, Hu XC, J. Appl. Polym. Sci., 101(2), 961 (2006)

[11] Jin HJ, Chen J, Karageorgiou V, Altman GH, Kaplan DL, Biomaterials, 25, 1039 (2004)

[12] Lee KH, Ki CS, Baek DH, Kang GD, Ihm DW, Park YH, Fibers Polym., 6, 181 (2005)

[13] Li C, Vepari C, Jin HJ, Kim HJ, Kaplan DL, Biomaterials, 27, 3115 (2006)

[14] Kang M, Jung R, Kim HS, Youk JH, Jin HJ, J. Nanosci. Nanotechnol., 7, 3888 (2007)

[15] Li WL, Wang JJ, Chi HX, Wei GZ, Zhang J, Dai LX, J. Appl. Polym. Sci., 123(1), 20 (2012)

[16] Kang M, Jin HJ, Colloid Polym. Sci., 285, 1163 (2007)

[17] Zhang YZ, Venugopal J, Huang ZM, Lim CT, Ramakrishna S, Polymer, 47(8), 2911 (2006)

[18] Chun JY, Kang HK, Jeong L, Kang YO, Oh JE, Yeo IS, Jung SY, Park WH, Min BM, Colloid Surf. B: Biointerfaces, 78, 334 (2010)

[19] Min BM, Lee G, Kim SH, Nam YS, Lee TS, Park WH, Biomaterials, 25, 1289 (2004)

[20] Mould AP, Askari JA, Humphries MJ, J. Biol. Chem., 275, 20324 (2000)

[21] Romanyuk A, Oelhafen P, Sol. Energy Mater. Sol. Cells, 91(12), 1051 (2007)

[22] Pal T, Curr. Sci., 83, 627 (2002)

[23] Atiyeh BS, Costagliola M, Hayek SN, Dibo SA, Burns, 33, 139 (2007)

[24] Poon VK, Burd A, Burns, 30, 140 (2004)

[25] Hidalgo E, Bartolome R, Barroso C, Moreno A, Dominguez C, Skin Pharmacol. Physiol., 11, 140 (1998)

[26] Hidalgo E, Domlnguez C, Toxicol. Lett., 98, 169 (1998)