Chitosan-based bioglass composite for bone tissue healing: Oxidative stress status and antiosteoporotic performance in a ovariectomized rat model

Samira Jebahi; Hassane Oudadesse; Gada Ben Saleh; Mongi Saoudi; Sirrar Mesadhi; Tarek Rebai; Hassib Keskes; Abdelfattah el Feki; Hafed el Feki

Korean Journal of Chemical Engineering, Vol.31, No.9, 1616-1623, September, 2014

DOI10.1007/s11814-014-0072-9 Export Citation

Chitosan-based bioglass composite for bone tissue healing: Oxidative stress status and antiosteoporotic performance in a ovariectomized rat model

Samira Jebahi^{1, 2, †}, Hassane Oudadesse¹, Gada Ben Saleh³, Mongi Saoudi², Sirrar Mesadhi⁴, Tarek Rebai⁴, Hassib Keskes⁴, Abdelfattah el Feki², and Hafed el Feki⁵

¹University of Rennes 1, UMR CNRS 6226, Campus de Beaulieu, 263 av. du Général Leclerc, 35042 Rennes, France
²Animal Ecophysiology Laboratory, Sfax Faculty of Science, Department of Life Sciences, Sfax, Tunisia
³Laboratory of Human Molecular Genetics, Faculty of Medicine, University of Sfax, Sfax, Tunisia
⁴Histology, Orthopaedic and Traumatology Laboratory, Sfax Faculty of Medicine, Sfax, Tunisia
⁵Science Materials and Environement Laboratory, Sfax Faculty of Science, Sfax, Tunisia

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Tissue engineering has opened up a new therapeutic avenue promising a revolution in regenerative medicine. Considerable attention has been given to chitosan composite materials and their applications in the field of the bone graft substitutes. We evaluated the antioxidative properties of chitosan-doped bioactive glass (BG-CH) with 17 wt% chitosan, and their applications in the guided bone regeneration. BG-CH was produced by a freeze-drying process and implanted in the femoral condyles of ovariectomized rats. Grafted bone tissues were carefully removed to evaluate the oxidative stress analysis, histomorphometric profile and mineral bone distribution by using inductively coupled plasma optical emission spectrometry (ICP-OES). A significant decrease of thiobarbituric acid-reactive substances (TBARs) was observed after BG-CH implantation. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities significantly increased in ovariectomized group implanted with chitosan-doped bioactive glass (OVXBG-CH) as compared to ovariectomized group implanted with bioactive glass (OVX-BG). The histomorphometric analysis showed that bone/tissue volume (BV/TV), osteoblast number (N.Ob) and osteoblast surface/bone surface (Ob.S/BS) were significantly higher in OVX-BG-CH group than in OVX-BG group. On the other hand, a rise in Ca and P ion concentrations in the implanted microenvironment was shown to lead to the ormation/deposition of Ca-P phases. Trace elements such as Sr and Fe were detected in the newly formed bone and involved in bone healing. These results suggested that BG-CH composites could become clinically useful as a therapeutic and implantable material.

Keywords:Chitosan;Bioactive Glass;Graft Biomaterial;Osteoporosis;Antioxidative Profile;Bone Regeneration

[References]

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[Referenced By]

[1] Tan G, Onur MA, Saglam N, Turk. J. Biol., 36, 621 (2012)

[2] Lee MJ, Seo DY, Lee HE, Choi GJ, Korean J. Chem. Eng., 28, 1411 (2011)

[3] Hench LL, Paschall HA, J. Biomed. Mater. Res., 7, 42 (1973)

[4] Ul-Islam M, Shah N, Ha JH, Park JK, Korean J. Chem. Eng., 28, 1743 (2011)

[5] Dasha M, Chiellinia F, Ottenbriteb RM, Chiellinia E, Prog. Polym. Sci., 36, 1014 (2011)

[6] Majeti NV, Kumar R, React. Funct. Polym., 46, 27 (2000)

[7] Jarudilokkul S, Tongthammachat A, Boonamnuayvittaya V, Korean J. Chem. Eng., 28, 1251 (2011)

[8] Iwata H, Yana S, Nasu M, Yosue T, Oral. Radiol., 21, 22 (2005)

[9] Kucukalic-Selimovic E, Valjevac A, Hadzovic-Dzuvo A, Skopljak-Beganovic A, Alimanovic-Alagic R, Brkovic A, Bosn. J. Basic. Med. Sci., 11, 5 (2011)

[10] Song KE, Min YK, Lee JK, Lee KB, Joo HJ, Kwack KS, Chung YS, Curr. Therap. Res., 69, 36 (2008)

[11] Rochet N, Balaguer T, Boukhechba F, Laugier JP, Quincey D, Goncalves S, Carle GF, Biomaterials, 30, 7 (2009)

[12] Luz GM, Mano JF, Biomed. Mater., 7, 054104 (2012)

[13] Mota J, Yu N, Caridade SG, Luz GM, Gomes ME, Reis RL, Jansen JA, Walboomers XF, Mano JF, Acta Biomater., 8, 80 (2012)

[14] Belalia R, Grelier S, Benaissa M, Coma V, Agric. Food Chem., 56, 8 (2008)

[15] Al-Deyab SS, Newehy MHE, Nirmala R, Abdel-Megeed A, Kim HY, Korean J. Chem. Eng., 30, 428 (2013)

[16] Bui XV, Oudadess H, Le Gal Y, Merdrignac-Conanec O, Cathelineau G, Korean J. Chem. Eng., 29, 220 (2012)

[17] Oudadesse H, Bui XV, Le Gal Y, Mostafaand A, Cathelineau G, Int. J. Biol. Biomed. Eng., 5, 56 (2011)

[18] Russell TT, Maran A, Lotinun S, Hefferan T, Evans GL, Zhang M, Rev. Endocr. Metab. Dis., 2, 127 (2001)

[19] Lam KK, Cheng PY, Hsiao G, Chen SY, Shen HH, Yen MH, Lee YM, Am. J. Hypertens., 22, 34 (2009)

[20] Kurutas EB, Sahan A, ltun TA, Turk. J. Biol., 33, 282 (2009)

[21] Tian F, Liu Y, Hu K, Zhao B, J. Mater. Sci., 38, 4712 (2003)

[22] Oudadesse H, Dietrich E, Gal YL, Pellen P, Bureau B, AA Mostafa, G Cathelineau, Biomed. Mater., 6, 035006 (2011)

[23] Buege JA, Aust SD, Methods Enzymol., 105, 310 (1984)

[24] Marklund S, Marklund G, Eur. J. Biochem., 47, 474 (1975)

[25] Pagila DE, Valentine WN, J. Lab. Clin. Med., 70, 169 (1967)

[26] Aebi H, Methods Enzymol., 105, 126 (1984)

[27] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ, J. Biol. Chem., 193, 275 (1951)

[28] Schenk RK, Merz WA, Muller JA, Acta Anat., 74, 53 (1969)

[29] Lean JM, Davies JT, Christopher KF, Kirstein JBG, Partington A, Urry ZL, Chambers TJ, Am. Soc. Clin. Invest., 112, 923 (2003)

[30] Muthusam S, Ramachandran I, Muthusamy B, Vasudevan G, Prabhu V, Subramaniam V, Jagadeesan A, Narasimhan S, Clin. Chim. Acta, 360, 6 (2005)

[31] Shahidi F, Arachchi JKV, Jeon YJ, Trends. Food Sci. Technol., 10, 51 (1999)

[32] Rashid TU, Rahman MM, Kabir S, Shamsuddin SM, Khan MA, Polym Int., 61, 1308 (2012)

[33] Chung YC, Kuo CL, Chen CC, Bio Technol., 96, 1482 (2005)

[34] Del Maestro RF, Bjork J, Arfors KE, Microvasc Res., 22, 70 (1981)

[35] Regnstrom K, Ragnarsson EG, Fryknas M, Koping-Hoggard M, Artursson P, Pharm. Res., 23, 482 (2006)

[36] Mate-Sanchez JEdV, Mazon P, Guirado JL, Ruiz RA, Negri MPB, Abboud M, De Aza PN, J. Biomed. Mater. Res. A. [Epub ahead of print] (2013)

[37] Arya N, Sardana V, Saxena M, Rangarajan A, Katti A, J. R. Soc. Interface.,[Epub ahead of print] (2012)

[38] Doblare M, Garcia JM, Acta Cient. Venez., 54, 58 (2003)

[39] Mente PL, Lewis JL, J. Orthop. Res., 7, 461 (1989)

[40] Verrona E, Gauthiera O, Janvierc P, Pileta P, Lesoeura J, Bujolic B, Guicheux J, Bouler JM, Biomaterials, 31, 84 (2010)

[41] Rahaman MN, Day DE, Bal BS, Fu Q, Jung SB, Bonewald LF, Tomsia AP, Acta Biomat., 7, 2373 (2011)

[42] Jebahi S, Oudadesse H, Feki HE, Rebai T, Keskes H, Pellen P, Feki AE, J. Appl. Biomed., 10, 209 (2012)

[43] Jayakumar R, Prabaharan M, Sudheesh Kumar PT, Nair SV, Tamura H, Biotechnol. Adv., 29, 37 (2011)

[44] Denuziere A, Ferrier D, Domard A, Ann. Pharm., 58, 53 (2000)