Amongst the Ti alloys used as orthopedic implant materials, Ti6Al4V is one of the widely used alloys. Magnetron sputtering was used to deposit nanocomposite coating of Ti-Si-Non the Ti6Al4V substrate at different power and then the coating structure and surface properties were characterized through contact angle measurement, X-ray diffraction (XRD), scanning electron microscopy (SEM), and atomic force microscopy (AFM). In vitro biocompatibility of the coatings was assessed by using mouse bone marrow mesenchymal stem cells (mBMMSC). Antibacterial studies were performed using Escherichia coli (E. coli) microorganisms. The osteogenic differentiation was also carried out in order to get gene expressions. The AFM results confirmed that the coatings deposited at 120W was smoother as compared to other coatings developed at different power, along with optimum contact angle, also these coatings showed good antibacterial results. The fluorescent and viability results of 120W sample confirmed their good biocompatibility as compared to the coatings deposited 20, 40, 60, and 100W power. Hence, the coating deposited at 120W exhibit desirable microstructural characteristics beneficial for surface modification of orthopedic implants. (C) 2013 Elsevier B.V. All rights reserved.