The surface of glass substrate was plasma treated, coated by gold nano-structures and subsequently grafted with nanoparticles. The samples were plasma treated, sputtered with Au nanostructures which was followed by grafting with biphenyl-4,41-dithiol (BPD) and then gold nanoparticles. The wettability, optical and chemical properties and surface morphology were studied. The adhesion and proliferation of vascular smooth muscle cells (VSMCs) on the samples were investigated in-vitro as well. Grafting of gold nanoparticles with the dithiol increases the UV-vis absorbance, the surface becomes more hydrophobic, rougher and more rugged compared to pristine, sputtered and only dithiol treated surface. Gold nanoparticles bound over dithiol and Au nanostructures cause better cell proliferation than purely BPD treated or pristine glass. C) 2014 Elsevier B.V. All rights reserved.