A novel one-pot multicomponent synthetic method of some new 2,4-disubstituted thiazoles catalyzed by enzyme was developed. Lipase from porcine pancreas (PPL) displayed good catalytic activity to promote this reaction starting from amines, aldehyde, thioacetic acid and methyl 3-(dimethyl amino)-2-isocyanoacrylate under mild conditions (35 A degrees C) with good yields (up to 93 %). The experimental results revealed PPL showed broad catalytic activity to substrates. The blank and control experiments revealed that PPL played important catalytic role during the process. All the new compounds were confirmed by IR, H-1 NMR, C-13 NMR, MS and elemental analysis. This PPL-catalyzed one-pot multicomponent synthetic method provided a new strategy to synthesize 2,4-disubstituted thiazoles and expanded the application of enzymes in organic synthesis.