Pure silica MFI-type materials were synthesized by a single step co-condensation approach using 3-mercaptopropyltrimethoxysilane (MPTS-Silicalite-1) as organo-functional compound and silica co-source. For comparison, MPTS-modified amorphous silica and Silicalite-1 templated with tetrapropylammonium hydroxide (TPA-Silicalite-1) were prepared. The incorporation of MPTS into the silica structures was confirmed by Si-29 and C-13 nuclear magnetic resonance (NMR) and Raman spectroscopy, while the porosity of the samples was verified with nitrogen sorption measurements. The MPTS-Silicalite-1 materials exhibit improved hydrophobicity in comparison with the samples prepared in the absence of MPTS. The presented method is simple and less toxic than the acid-meditated synthesis approach commonly applied for the preparation of hydrophobic zeolitic materials. (c) 2012 Elsevier B.V. All rights reserved.