Glucose oxidase enzyme was immobilized on ferroelectric LiNbO3 single crystals that were able to generate acoustic wave resonance oscillations by radio frequency electric power. The generation of the 3.6-14.7 MHz acoustic waves with lattice distortion vertical to the surface caused increases in the enzyme activity for glucose oxidation by decreasing the activation energy of reaction. A mechanism is proposed in which the acoustic waves enhance the interactions between the glucose substrate and the enzyme.